ArticleRegenerative biomaterials2021
Immunological and functional features of decellularized xenogeneic heart valves after transplantation into GGTA1-KO pigs.
Article in Regenerative biomaterials, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 22 citations in OpenAlex.
- Tissue Preservation and Access: Modern Innovation in Biobanking Moving Forwards a Personalized Treatment.Journal of personalized medicine · 2025Review
- Semi-quantitative scoring criteria based on multiple staining methods combined with machine learning to evaluate residual nuclei in decellularized matrix.Regenerative biomaterials · 2025Article
- Advances in xenogeneic donor decellularized organs: A review on studies with sheep and porcine-derived heart valves.Bioengineering & translational medicine · 2024Review
- Matched comparison of decellularized homografts and bovine jugular vein conduits for pulmonary valve replacement in congenital heart disease.Cell and tissue banking · 2024Article
- Regulatory science promotes the translation of transcatheter tricuspid valve repair/replacement devices.Regenerative biomaterials · 2024Article
- Effects of the matrix-bounded nanovesicles of high-hydrostatic pressure decellularized tissues on neural regeneration.Science and technology of advanced materials · 2024Article
- Recent progress in functional modification and crosslinking of bioprosthetic heart valves.Regenerative biomaterials · 2024Review
- Sacrificial scaffold-assisted direct ink writing of engineered aortic valve prostheses.Biofabrication · 2023Article
- Characterization of a Decellularized Sheep Pulmonary Heart Valves and Analysis of Their Capability as a Xenograft Initial Matrix Material in Heart Valve Tissue Engineering.Bioengineering (Basel, Switzerland) · 2023Article
- 5-Year results from the prospective European multi-centre study on decellularized homografts for pulmonary valve replacement ESPOIR Trial and ESPOIR Registry data.European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery · 2022Article
- α-Gal antigen-deficient rabbits with GGTA1 gene disruption via CRISPR/Cas9.BMC genomic data · 2022Article
- Immunological Risk Assessment of Xenogeneic Dural Patch by Comparing with Raw Material via GTKO Mice.BioMed research international · 2022Article
- Recent advances in regenerative biomaterials.Regenerative biomaterials · 2022Review
- Serial assessment of early antibody binding to decellularized valved allografts.Frontiers in cardiovascular medicine · 2022Article
Corrections and comments
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Authors and funding
13 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Decellularization of xenogeneic heart valves might lead to excellent regenerative implants, from which many patients could benefit. However, this material carries various xenogeneic epitopes and thus bears a considerable inherent immunological risk. Here, we investigated the regenerative and immunogenic potential of xenogeneic decellularized heart valve implants using pigs deficient for the galactosyltransferase gene (GGTA1-KO) as novel large animal model. Decellularized aortic and pulmonary heart valves obtained from sheep, wild-type pigs or GGTA1-KO pigs were implanted into GGTA1-KO pigs for 3, or 6 months, respectively. Explants were analyzed histologically, immunhistologically (CD3, CD21 and CD172a) and anti-αGal antibody serum titers were determined by ELISA. Xenogeneic sheep derived implants exhibited a strong immune reaction upon implantation into GGTA1-KO pigs, characterized by massive inflammatory cells infiltrates, presence of foreign body giant cells, a dramatic increase of anti-αGal antibody titers and ultimately destruction of the graft, whereas wild-type porcine grafts induced only a mild reaction in GGTA1-KO pigs. Allogeneic implants, wild-type/wild-type and GGTA1-KO/GGTA1-KO valves did not induce a measurable immune reaction. Thus, GGTA1-KO pigs developed a 'human-like' immune response toward decellularized xenogeneic implants showing that immunogenicity of xenogeneic implants is not sufficiently reduced by decellularization, which detracts from their regenerative potential.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.