Evidence map›Paper›PMID 35928002›Full record

ArticleSTAR protocols2022

Monitoring condensate dynamics in

Macy L Sprunger, Meredith E Jackrel

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 58% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Macy L SprungerDepartment of Chemistry, Washington University, St. Louis, MO 63130, USA.
Meredith E JackrelDepartment of Chemistry, Washington University, St. Louis, MO 63130, USA. Electronic address: mjackrel@wustl.edu.
Washington University in St. Louis · US

Funding

Safeguards of the Proteome: Elucidating the Roles of Protein DisaggregasesR35GM128772 · NIGMS · WASHINGTON UNIVERSITY · PI JACKREL, MEREDITH E. · 2018 to 2022
$2.0M
ZEISS LSM880 AIRYSCAN MICROSCOPE FOR SHARED BIOMEDICAL RESEARCHS10OD021629 · OD · WASHINGTON UNIVERSITY · PI FITZPATRICK, JAMES ALEXANDER · 2017 to 2017
$596k
Defining the molecular drivers and modulators of MATR3 proteinopathy implicated in amyotrophic lateral sclerosis and frontotemporal dementiaF31NS120512 · NINDS · WASHINGTON UNIVERSITY · PI SPRUNGER, MACY L · 2021 to 2023
$128k
NIGMS NIH HHS R35 GM128772NIH HHS S10 OD021629NINDS NIH HHS F31 NS120512
6 · The paper itself

Abstract

This protocol describes the use of fluorescence recovery after photobleaching (FRAP) to investigate the dynamics of Matrin-3 (MATR3) condensates in live budding yeast. We detail how to generate yeast strains containing MATR3 with an enhanced green fluorescent protein (eGFP) tag and induce MATR3-eGFP expression. We provide steps to prepare slides of immobilized yeast cells and perform FRAP imaging and data analysis. This protocol can be broadly applied to study condensate dynamics of a range of proteins in different model systems. For complete details on the use and execution of this protocol, please refer to Sprunger et al. (2022).

Indexed as

Saccharomyces cerevisiaeSaccharomycetalesFluorescence Recovery After PhotobleachingBiophysicsCell BiologyMicroscopyModel OrganismsProtein Biochemistry

Identifiers

PMID35928002
PMCPMC9344031
OpenAlexW4288949244

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.