Evidence map›Paper›PMID 35928001›Full record

ArticleSTAR protocols2022

Enrichment of aging yeast cells and budding polarity assay in

Emily J Yang, Liza A Pon

Abstract read
In one paragraph

Article in STAR protocols, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Emily J YangDepartment of Pathology and Cell Biology, Columbia University, New York, NY, USA. Electronic address: jy2907@cumc.columbia.edu.
Liza A PonDepartment of Pathology and Cell Biology, Columbia University, New York, NY, USA. Electronic address: lap5@cumc.columbia.edu.

Funding

Tumor Biology and Microenvironment ProgramP30CA013696 · NCI · COLUMBIA UNIV NEW YORK MORNINGSIDE · PI Anil K Rustgi · 1985 to 2026
$115.3M
Mitochondrial inheritance and quality controlR35GM122589 · NIGMS · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI Liza A Pon · 2017 to 2026
$5.1M
MITOCHONDRIAL MOTILITY AND INHERITANCER01GM045735 · NIGMS · COLUMBIA UNIV NEW YORK MORNINGSIDE · PI PON, LIZA A · 1993 to 2016
$4.2M
The Role of Actin in Cellular AgingR33AG051047 · NIA · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI PON, LIZA A · 2018 to 2020
$1.4M
The Role of Actin in Cellular AgingR21AG051047 · NIA · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI PON, LIZA A · 2016 to 2017
$427k
NCI NIH HHS P30 CA013696NIA NIH HHS R21 AG051047NIA NIH HHS R33 AG051047NIGMS NIH HHS R01 GM045735NIGMS NIH HHS R35 GM122589
6 · The paper itself

Abstract

Replicative lifespan, a measure of the number of times that a yeast cell can divide before senescence, is one model for aging. Here, we provide a protocol for enrichment of yeast as a function of replicative age using a miniature chemostat aging device (mCAD). This protocol allows for isolation of quantities of cells that are sufficient for biochemical or genomic analysis. We also describe an approach to assess bud site selection, a marker for cell polarity, during the aging process. For complete details on the use and execution of this protocol, please refer to Yang et al. (2022).

Indexed as

Saccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsCell DivisionCellular SenescenceSaccharomyces cerevisiae ProteinsCell-based AssaysCell BiologyCell isolationMicroscopyModel Organisms

Identifiers

PMID35928001
PMCPMC9344026

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.