ArticleBiophysical journal2022
Distribution of cholesterol in asymmetric membranes driven by composition and differential stress.
Article in Biophysical journal, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Does Complexation of Plasma Membrane Cholesterol with Phospholipids Determine its Transbilayer Distribution?The Journal of membrane biology · 2026Article
- A Spectroelectrochemical Study of the Effect of Asymmetry on the Electrochemical Response of Lipid Bilayers.The journal of physical chemistry. B · 2026Article
- Critique of a Radically New Model for Plasma Membrane Bilayer Organization.BioEssays : news and reviews in molecular, cellular and developmental biology · 2026Review
- Asymmetry and heterogeneity in the plasma membrane.Biophysical journal · 2026Article
- A dynamic feedback loop between retrograde sterol transport and TORC2 controls adaptation of the plasma membrane to stress.The EMBO journal · 2025Article
- Bottom-up Coarse-Grained Models of Asymmetric Membranes.The journal of physical chemistry. B · 2025Article
- Cholesterol-targeting Wnt-β-catenin signaling inhibitors for colorectal cancer.Nature chemical biology · 2025Article
- Review
- Elucidating the mechanical properties of asymmetric membranes by direct derivation of their energetics.Faraday discussions · 2025Article
- Comprehensive Insights into the Cholesterol-Mediated Modulation of Membrane Function Through Molecular Dynamics Simulations.Membranes · 2025Review
- Article
- Role of Protein Regulators of Cholesterol Homeostasis in Immune Modulation and Cancer Pathophysiology.Endocrinology · 2025Review
- Lipid Membrane Leaflets Unzip upon Hybridization with Polymer-Rich Nanodomains.Macromolecules · 2024Article
- Unexpected asymmetric distribution of cholesterol and phospholipids in equilibrium model membranes.Biophysical journal · 2024Article
- A tuneable minimal cell membrane reveals that two lipid species suffice for life.Nature communications · 2024Article
- A tuneable minimal cell membrane reveals that two lipid species suffice for life.bioRxiv : the preprint server for biology · 2024Article
- Spontaneous curvature generation by peptides in asymmetric bilayers.Journal of computational chemistry · 2024Article
- Seeing the Membrane from Both Sides Now: Lipid Asymmetry and Its Strange Consequences.Cold Spring Harbor perspectives in biology · 2023Review
- The asymmetric plasma membrane-A composite material combining different functionalities?: Balancing Barrier Function and Fluidity for Effective Signaling.BioEssays : news and reviews in molecular, cellular and developmental biology · 2023Article
- Efficient Simulations of Solvent Asymmetry Across Lipid Membranes Using Flat-Bottom Restraints.Journal of chemical theory and computation · 2023Article
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2 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Many lipid membranes of eukaryotic cells are asymmetric, which means the two leaflets differ in at least one physical property, such as lipid composition or lateral stress. Maintaining this asymmetry is helped by the fact that ordinary phospholipids rarely transition between leaflets, but cholesterol is an exception: its flip-flop times are in the microsecond range, so that its distribution between leaflets is determined by a chemical equilibrium. In particular, preferential partitioning can draw cholesterol into a more saturated leaflet, and phospholipid number asymmetry can force it out of a compressed leaflet. Combining highly coarse-grained membrane simulations with theoretical modeling, we investigate how these two driving forces play against each other until cholesterol's chemical potential is equilibrated. The theory includes two coupled elastic sheets and a Flory-Huggins mixing free energy with a χ parameter. We obtain a relationship between χ and the interaction strength between cholesterol and lipids in either of the two leaflets, and we find that it depends, albeit weakly, on lipid number asymmetry. The differential stress measurements under various asymmetry conditions agree with our theoretical predictions. Using the two kinds of asymmetries in combination, we find that it is possible to counteract the phospholipid number bias, and the resultant stress in the membrane, via the control of cholesterol mixing in the leaflets.
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