ArticleNature chemistry2022
Standardized excitable elements for scalable engineering of far-from-equilibrium chemical networks.
Article in Nature chemistry, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Continuously tunable multistability in DNA replication networks.Nature communications · 2026Article
- Dynamic Control of DNA Origami Self-Assembly by Transcriptional Modules.Journal of the American Chemical Society · 2026Article
- Concept, development and applications of DNA computation.Fundamental research · 2026Review
- DNA computing function switching by programming base stacking interactions with minimal molecular architecture changes.Nature communications · 2025Article
- Article
- Cell-Membrane-Anchored Synthetic Dynamic DNA Circuits for Signaling Transient Cell Migration.Journal of the American Chemical Society · 2025Article
- Diversifying Substrates and Reaction Conditions for Polymerase Strand Recycling.ACS synthetic biology · 2025Article
- An enzyme-based approach for highly efficient self-replication of DNA origami dimers.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- RNA origami nanotubes push the boundaries of confinement.Nature nanotechnology · 2025Article
- Spatial Control over Reactions via Localized Transcription within Membraneless DNA Nanostar Droplets.Journal of the American Chemical Society · 2024Article
- Directing Nanoparticle Organization in Response to Diverse Chemical Inputs.Journal of the American Chemical Society · 2024Article
- Developmental assembly of multi-component polymer systems through interconnected synthetic gene networks in vitro.Nature communications · 2024Article
- Y-switch: a spring-loaded synthetic gene switch for robust DNA/RNA signal amplification and detection.Nucleic acids research · 2024Article
- Plug-and-play protein biosensors using aptamer-regulated in vitro transcription.Nature communications · 2024Article
- Understanding the relationship between sequences and kinetics of DNA strand displacements.Nucleic acids research · 2024Article
- Programming gel automata shapes using DNA instructions.Nature communications · 2024Article
- Strategies to Reduce Promoter-Independent Transcription of DNA Nanostructures and Strand Displacement Complexes.ACS synthetic biology · 2024Review
- Engineering a cell-free biosensor signal amplification circuit with polymerase strand recycling.bioRxiv : the preprint server for biology · 2024Article
- DNA as a universal chemical substrate for computing and data storage.Nature reviews. Chemistry · 2024Review
- Programmable Biomolecule-Mediated Processors.Journal of the American Chemical Society · 2023Review
Corrections and comments
- Erratum issued
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Engineered far-from-equilibrium synthetic chemical networks that pulse or switch states in response to environmental signals could precisely regulate the kinetics of chemical synthesis or self-assembly. Currently, such networks must be extensively tuned to compensate for the different activities of and unintended reactions between a network's various chemical components. Modular elements with standardized performance could be used to rapidly construct networks with designed functions. Here we develop standardized excitable chemical regulatory elements, termed genelets, and use them to construct complex in vitro transcriptional networks. We develop a protocol for identifying >15 interchangeable genelet elements with uniform performance and minimal crosstalk. These elements can be combined to engineer feedforward and feedback modules whose dynamics match those predicted by a simple kinetic model. Modules can then be rationally integrated and organized into networks that produce tunable temporal pulses and act as multistate switchable memories. Standardized genelet elements, and the workflow to identify more, should make engineering complex far-from-equilibrium chemical dynamics routine.
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