ArticleJournal of experimental botany2022
The Sclerotinia sclerotiorum-inducible promoter pBnGH17D7 in Brassica napus: isolation, characterization, and application in host-induced gene silencing.
Article in Journal of experimental botany, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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8 citing papers in PubMed, 20 citations in OpenAlex.
- Virus-Inducible Activation of Hypersensitive Response Confers Broad-Spectrum Resistance to Diverse Viruses in Plants.Molecular plant pathology · 2025Article
- Transgenic validation of a promoter strongly inducible by Agrobacterium tumefaciens.Scientific reports · 2025Article
- The transcription factor BnaWRKY75 enhances resistance to the necrotrophic pathogen Sclerotinia sclerotiorum by promoting salicylic acid biosynthesis in oilseed rape.Journal of experimental botany · 2025Article
- Antimicrobial Peptides: Classification, Mechanism, and Application in Plant Disease Resistance.Probiotics and antimicrobial proteins · 2025Review
- A GDSL motif-containing lipase modulates Sclerotinia sclerotiorum resistance in Brassica napus.Plant physiology · 2024Article
- Natural variation in BnaA07.MKK9 confers resistance to Sclerotinia stem rot in oilseed rape.Nature communications · 2024Article
- Genetic breakthroughs in theFrontiers in plant science · 2023Review
- Fungal effectors versus defense-related genes ofFrontiers in plant science · 2023Review
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Authors and funding
10 authors at 1 institution in 1 country.
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Abstract
Sclerotinia stem rot (SSR), caused by Sclerotinia sclerotiorum, is among the most devastating diseases in Brassica napus worldwide. Conventional breeding for SSR resistance in Brassica species is challenging due to the limited availability of resistant germplasm. Therefore, genetic engineering is an attractive approach for developing SSR-resistant Brassica crops. Compared with the constitutive promoter, an S. sclerotiorum-inducible promoter would avoid ectopic expression of defense genes that may cause plant growth deficits. In this study, we generated a S. sclerotiorum-inducible promoter. pBnGH17D7, from the promoter of B. napus glycosyl hydrolase 17 gene (pBnGH17). Specifically, 5'-deletion and promoter activity analyses in transgenic Arabidopsis thaliana plants defined a 189 bp region of pBnGH17 which was indispensable for S. sclerotiorum-induced response. Compared with pBnGH17, pBnGH17D7 showed a similar response upon S. sclerotiorum infection, but lower activity in plant tissues in the absence of S. sclerotiorum infection. Moreover, we revealed that the transcription factor BnTGA7 directly binds to the TGACG motif in pBnGH17D7 to activate BnGH17. Ultimately, pBnGH17D7 was exploited for engineering Sclerotinia-resistant B. napus via host-induced gene silencing. It induces high expression of siRNAs against the S. sclerotiorum pathogenic factor gene specifically during infection, leading to increased resistance.
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