ArticleComputers in biology and medicine2022
Single cell gene expression profiling of nasal ciliated cells reveals distinctive biological processes related to epigenetic mechanisms in patients with severe COVID-19.
Article in Computers in biology and medicine, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed, 6 citations in OpenAlex.
- Beyond the canonical sequence: IsomiRs as modulators and drivers of MASLD progression: Editorial on "MicroRNA isomiRs reveal novel pathways linked to disease activity and fibrosis in MASLD".Clinical and molecular hepatology · 2026Article
- Host transcriptomic analysis reveals a defective intracellular environment that limits SARS-CoV-2 replication in CFTR-deficient airway epithelium.Frontiers in cellular and infection microbiology · 2026Article
- Single cell characterization of blood and expanded regulatory T cells in autoimmune polyendocrine syndrome type 1.iScience · 2024Article
- Cell annotation using scRNA-seq data: A protein-protein interaction network approach.MethodsX · 2023Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
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Abstract
objectiveTo explore the molecular processes associated with cellular regulatory programs in patients with COVID-19, including gene activation or repression mediated by epigenetic mechanisms. We hypothesized that a comprehensive gene expression profiling of nasopharyngeal epithelial cells might expand our understanding of the pathogenic mechanisms of severe COVID-19.
methodsWe used single-cell RNA sequencing (scRNAseq) profiling of ciliated cells (n = 12,725) from healthy controls (SARS-CoV-2 negative n = 13) and patients with mild/moderate (n = 13) and severe (n = 14) COVID-19. ScRNAseq data at the patient level were used to perform gene set and pathway enrichment analyses. We prioritized candidate miRNA-target interactions and epigenetic mechanisms.
resultsWe found that mild/moderate COVID-19 compared to healthy controls had upregulation of gene expression signatures associated with mitochondrial function, misfolded proteins, and membrane permeability. In addition, we found that compared to mild/moderate disease, severe COVID-19 had downregulation of epigenetic mechanisms, including DNA and histone H3K4 methylation and chromatin remodelling regulation. Furthermore, we found 11-ranked miRNAs that may explain miRNA-dependent regulation of histone methylation, some of which share seed sequences with SARS-CoV-2 miRNAs.
conclusionOur results may provide novel insights into the epigenetic mechanisms mediating the clinical course of SARS-CoV-2 infection.
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