Evidence map›Paper›PMID 35925447›Full record

ArticleIn vitro cellular & developmental biology. Animal2022

Linoleic and linolenic acids reduce the effects of heat stress-induced damage in pig oocytes during maturation in vitro.

Jaelyn Z Current, Miranda Mentler, Brian D Whitaker

Abstract read
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In one paragraph

Article in In vitro cellular & developmental biology. Animal, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed, 1 pooled it
1.6field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 1 synthesis or guideline pooled it, 8 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Jaelyn Z CurrentDepartment of Animal and Pre-Veterinary Studies, University of Findlay, 1000 North Main Street, Findlay, OH, 45840, USA.
Miranda MentlerDepartment of Animal and Pre-Veterinary Studies, University of Findlay, 1000 North Main Street, Findlay, OH, 45840, USA.
Brian D WhitakerDepartment of Animal and Pre-Veterinary Studies, University of Findlay, 1000 North Main Street, Findlay, OH, 45840, USA. whitaker@findlay.edu.ORCID http://orcid.org/0000-0001-8032-0845
University of Findlay · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Elevated environmental temperatures can induce heat stress which could reduce fertility and early embryonic development. Fatty acids can initiate an endergonic reaction that absorbs cellular heat and decreases intracellular temperature. This study's objective was to minimize heat stress-induced damage to in vitro matured oocytes by supplementing maturation media with either 50 μM linoleic or linolenic acid or both (25 or 50 μM) during maturation at either 38.5 or 41.5°C. Oocytes were evaluated for intracellular antioxidative pathways, fertilization characteristics, or early embryonic development. Elevated maturation temperatures increased (p < 0.05) reactive oxygen species (ROS) formation and supplementation with linoleic or linolenic acid decreased (p < 0.05) ROS in oocytes matured at 41.5°C. Maturation temperature had an effect (p < 0.05) on the intracellular antioxidative pathways of the oocyte except for glutathione peroxidase activity. Regardless of maturation temperature, supplementation with linoleic or linolenic acid increased (p < 0.05) the enzyme activities and glutathione concentrations in the oocytes compared to no fatty acid supplementation. Supplementation of both linoleic and linolenic acid decreased (p < 0.05) polyspermic fertilization rates. Supplementing either 25 or 50 μM linoleic and linolenic acid to maturing oocytes at 41.5°C increased (p < 0.05) cleavage rates by 48 h after IVF and the blastocyst formation rates by 144 h after IVF compared to other treatments. Oocytes matured at 38.5°C had greater (p < 0.05) embryonic development than those matured at 41.5°C except for those supplemented with 50 μM linoleic and linolenic acid. Supplementing 50 μM linoleic and linolenic acid to the maturation medium of pig oocytes reduces the effects of heat stress-induced damage.

Indexed as

In Vitro Oocyte Maturation TechniquesLinolenic AcidsAnimalsAntioxidantsBlastocystEmbryonic DevelopmentFertilization in VitroGlutathioneGlutathione PeroxidaseHeat-Shock ResponseOocytesReactive Oxygen SpeciesSwineAntioxidantsGlutathioneGlutathione PeroxidaseLinolenic AcidsReactive Oxygen SpeciesHeat stressLinoleic acidLinolenic acidOocyte maturation

Identifiers

PMID35925447
OpenAlexW4289780583

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.