ArticleJournal of cardiovascular electrophysiology2022
Establishing electroporation thresholds for targeted cell specific cardiac ablation in a 2D culture model.
Article in Journal of cardiovascular electrophysiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 23 citations in OpenAlex.
- Targeted ablation of epicardial ganglionated plexi during cardiac surgery with pulsed field electroporation (NEURAL AF).Journal of interventional cardiac electrophysiology : an international journal of arrhythmias and pacing · 2025Trial
- Biophysical principles of pulsed field ablation.Europace : European pacing, arrhythmias, and cardiac electrophysiology : journal of the working groups on cardiac pacing, arrhythmias, and cardiac cellular electrophysiology of the European Society of Cardiology · 2026Review
- Computational Assessment of Electrical and Thermal Effects of Epicardial Pulsed Field Ablation Adjacent to Stented Arteries.Bioengineering (Basel, Switzerland) · 2026Article
- Role of cell density and proximity in electroporation for tissue ablation.Communications physics · 2026Article
- The Clinical Utility of 3D Electroanatomical Mapping for Atrial Fibrillation Ablation by Pulsed Field Ablation.Journal of arrhythmia · 2025Article
- High-Throughput Capable Three-Dimensional Tissue Model for Quantification of Electroporation Thresholds.Journal of visualized experiments : JoVE · 2025Article
- Effects of electric field and heat distribution due to trends in metal stent diameter in epicardial pulsed field ablation.Scientific reports · 2025Article
- Complexity and Perplexity of Pulsed Field Ablation: An Engineering Perspective.Arrhythmia & electrophysiology review · 2025Review
- Feasibility study of intravascular pulsed electric field ablation for the treatment of cardiac arrhythmias.Frontiers in physiology · 2025Article
- Pulsed Field Ablation: A Comprehensive Update.Journal of clinical medicine · 2024Review
- The Intrinsic Cardiac Nervous System: From Pathophysiology to Therapeutic Implications.Biology · 2024Review
- Opportunities and Challenges in Catheter-Based Irreversible Electroporation for Ventricular Tachycardia.Pathophysiology : the official journal of the International Society for Pathophysiology · 2024Review
- Epicardial Pulsed Field Ablation of Ganglionated Plexi: Computational and Pre-Clinical Evaluation of a Bipolar Sub-Xiphoid Catheter for the Treatment of Atrial Fibrillation.Bioengineering (Basel, Switzerland) · 2023Article
- Cardioneuroablation Using Epicardial Pulsed Field Ablation for the Treatment of Atrial Fibrillation.Journal of cardiovascular development and disease · 2023Review
- Acute ATP loss during irreversible electroporation mediates caspase independent cell death.Bioelectrochemistry (Amsterdam, Netherlands) · 2023Article
- Microglia isolation from aging mice for cell culture: A beginner's guide.Frontiers in cellular neuroscience · 2023Article
- Determination of lethal electric field threshold for pulsed field ablation inFrontiers in cardiovascular medicine · 2023Article
- Pulsed Electric Field Ablation of Epicardial Autonomic Ganglia: Computer Analysis of Monopolar Electric Field across the Tissues Involved.Bioengineering (Basel, Switzerland) · 2022Article
- Establishing electroporation thresholds for targeted cell specific cardiac ablation in a 2D culture model.Journal of cardiovascular electrophysiology · 2022Article
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Authors and funding
10 authors at 5 institutions in 2 countries.
Funding
Abstract
backgroundIrreversible electroporation has emerged as a new modality to overcome issues associated with other energy sources for cardiac ablation. Strong evidence on the optimal, effective, and selective voltage threshold is lacking for both in vitro and preclinical in vivo studies. The aim of this study is to examine the optimal threshold for selective cell ablation on cardiac associated cell types.
methodsConventional monophasic and biphasic pulses of different field strength were delivered in a monolayer culture system of cardiomyocytes, neurons, and adipocytes. The dynamics of cell death mechanisms were examined at different time points.
resultsNeurons exhibit higher susceptibility to electroporation and cell death at higher field strength of 1250 V/cm in comparison to cardiomyocytes. Cardiac adipocytes showed lower susceptibility to electroporation in comparison to other cell types. A significant proportion of cardiomyocytes recovered after 24 h postelectroporation, while neuronal cell death remained consistent but with a significant delayed cell death at a higher voltage threshold. Caspase 3/7 activity was observed in both cardiomyocytes and neurons, with a higher level of activity in cardiomyocytes in response to electroporation. Biphasic and monophasic pulses showed no significant difference in both cell types, and significantly lower cell death in neurons when inter pulse interval was reduced.
conclusionsThis study presents important findings on the differences in the susceptibility of neurons and cardiomyocytes to irreversible electroporation. Cell type alone yielded selective and different dynamics in terms of the evolution and signaling mechanism of cell death in response to electroporation.
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