Evidence map›Paper›PMID 35923689›Full record

ReviewFrontiers in genetics2022

New Hope for Genome Editing in Cultivated Grasses: CRISPR Variants and Application.

Asad Riaz, Farah Kanwal, Iqrar Ahmad, Shakeel Ahmad, Ayesha Farooq, Claus Krogh Madsen, Henrik Brinch-Pedersen, Zelalem Eshetu Bekalu, Fei Dai, Guoping Zhang and 1 more

Open access · goldAbstract readReview
In one paragraph

Review in Frontiers in genetics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
1.5field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 19 citations in OpenAlex.

  1. Article
  2. CRISPR/Cas system-mediated transgene-free or DNA-free genome editing in plants.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025
    Review
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  7. Article
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  11. Phenotypic and genetic characterization of anFrontiers in plant science · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 4 institutions in 3 countries.

Asad RiazCollege of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China.
Farah KanwalCollege of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China.
Iqrar AhmadCentre for Advanced Studies in Agriculture and Food Security, University of Agriculture Faisalabad, Faisalabad, Pakistan.
Shakeel AhmadCentre for Advanced Studies in Agriculture and Food Security, University of Agriculture Faisalabad, Faisalabad, Pakistan.
Ayesha FarooqCentre for Advanced Studies in Agriculture and Food Security, University of Agriculture Faisalabad, Faisalabad, Pakistan.
Claus Krogh MadsenDepartment of Agroecology, Research Center Flakkebjerg, Aarhus University, Slagelse, Denmark.
Henrik Brinch-PedersenDepartment of Agroecology, Research Center Flakkebjerg, Aarhus University, Slagelse, Denmark.
Zelalem Eshetu BekaluDepartment of Agroecology, Research Center Flakkebjerg, Aarhus University, Slagelse, Denmark.
Fei DaiCollege of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China.
Guoping ZhangCollege of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China.
Ahmad M AlqudahDepartment of Agroecology, Research Center Flakkebjerg, Aarhus University, Slagelse, Denmark.
Aarhus University · DKZhejiang University · CNUniversity of Faisalabad · PKUniversity of Agriculture Faisalabad · PK

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

With the advent of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated protein (Cas) mediated genome editing, crop improvement has progressed significantly in recent years. In this genome editing tool, CRISPR-associated Cas nucleases are restricted to their target of DNA by their preferred protospacer adjacent motifs (PAMs). A number of CRISPR-Cas variants have been developed e.g. CRISPR-Cas9, -Cas12a and -Cas12b, with different PAM requirements. In this mini-review, we briefly explain the components of the CRISPR-based genome editing tool for crop improvement. Moreover, we intend to highlight the information on the latest development and breakthrough in CRISPR technology, with a focus on a comparison of major variants (CRISPR-Cas9, -Cas12a, and -Cas12b) to the newly developed CRISPR-SpRY that have nearly PAM-less genome editing ability. Additionally, we briefly explain the application of CRISPR technology in the improvement of cultivated grasses with regard to biotic and abiotic stress tolerance as well as improving the quality and yield.

Indexed as

Cas12Cas9CRISPRcultivated-grassesplant genome editingSPRYstress tolerance

Identifiers

PMID35923689
PMCPMC9340155
OpenAlexW4285800553

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.