Evidence map›Paper›PMID 35922747›Full record

ArticleBMC genomics2022

Circular RNA expression profiles and CircSnd1-miR-135b/c-foxl2 axis analysis in gonadal differentiation of protogynous hermaphroditic ricefield eel Monopterus albus.

Zhi He, Zhijun Ma, Deying Yang, Qiqi Chen, Zhide He, Jiaxiang Hu, Faqiang Deng, Qian Zhang, Jiayang He, Lijuan Ye and 8 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC genomics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
3.9field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 11 citations in OpenAlex.

  1. Article
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  6. FoxH1 Represses the Promoter Activity ofInternational journal of molecular sciences · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors at 2 institutions in 1 country.

Zhi He *College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Zhijun Ma *College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Deying Yang *College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Qiqi ChenCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Zhide HeLuzhou City Department of Agricultural and Rural Affairs, Luzhou, 646000, Sichuan, China.
Jiaxiang HuSichuan Water Conservancy Vocational College, Chengdu, 611231, Sichuan, China.
Faqiang DengCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Qian ZhangCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Jiayang HeCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Lijuan YeCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Hongjun ChenCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Liang HeCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Xiaoli HuangCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Wei LuoCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Shiyong YangCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Xiaobin GuCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Mingwang ZhangCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Taiming YanCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. yantaiming@sicau.edu.cn.
Sichuan Agricultural University · CNHealth and Family Planning Commission of Sichuan Province · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe expression and biological functions of circular RNAs (circRNAs) in reproductive organs have been extensively reported. However, it is still unclear whether circRNAs are involved in sex change. To this end, RNA sequencing (RNA-seq) was performed in gonads at 5 sexual stages (ovary, early intersexual stage gonad, middle intersexual stage gonad, late intersexual stage gonad, and testis) of ricefield eel, and the expression profiles and potential functions of circRNAs were studied.

resultsSeven hundred twenty-one circRNAs were identified, and the expression levels of 10 circRNAs were verified by quantitative real-time PCR (qRT-PCR) and found to be in accordance with the RNA-seq data, suggesting that the RNA-seq data were reliable. Then, the sequence length, category, sequence composition and the relationship between the parent genes of the circRNAs were explored. A total of 147 circRNAs were differentially expressed in the sex change process, and GO and KEGG analyses revealed that some differentially expressed (such as novel_circ_0000659, novel_circ_0004005 and novel_circ_0005865) circRNAs were closely involved in sex change. Furthermore, expression pattern analysis demonstrated that both circSnd1 and foxl2 were downregulated in the process of sex change, which was contrary to mal-miR-135b. Finally, dual-luciferase reporter assay and RNA immunoprecipitation showed that circSnd1 and foxl2 can combine with mal-miR-135b and mal-miR-135c. These data revealed that circSnd1 regulates foxl2 expression in the sex change of ricefield eel by acting as a sponge of mal-miR-135b/c.

conclusionOur results are the first to demonstrate that circRNAs have potential effects on sex change in ricefield eel; and circSnd1 could regulate foxl2 expression in the sex change of ricefield eel by acting as a sponge of mal-miR-135b/c. These data will be useful for enhancing our understanding of sequential hermaphroditism and sex change in ricefield eel or other teleosts.

Indexed as

Disorders of Sex DevelopmentMicroRNAsSmegmamorphaAnimalsEelsFemaleGonadsMaleRNA, CircularMicroRNAsRNA, CircularcircRNAExpression patternFoxl2Gonadal differentiationMonopterus albusReproductive

Identifiers

PMID35922747
PMCPMC9347082
OpenAlexW4289517114

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.