Evidence map›Paper›PMID 35920441›Full record

ArticleEpigenetics2022

Precise epigenomic editing with a SunTag-based modular epigenetic toolkit.

Subhrangshu Guhathakurta, Levi Adams, Inhye Jeong, Anishaa Sivakumar, Mingyu Cha, Mariana Bernardo Fiadeiro, Haiyan Nancy Hu, Yoon-Seong Kim

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Epigenetics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Review
  2. Review
  3. Screening and Identification of Hnf1ba-International journal of molecular sciences · 2025
    Article
  4. Review
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Subhrangshu GuhathakurtaBurnett School of Biomedical Sciences, UCF College of Medicine, University of Central Florida, Orlando, FL USA.
Levi AdamsBurnett School of Biomedical Sciences, UCF College of Medicine, University of Central Florida, Orlando, FL USA.
Inhye JeongRobert Wood Johnson Medical School Institute for Neurological Therapeutics, and Department of Neurology, Rutgers Biomedical and Health Sciences, Piscataway, NJ USA.
Anishaa SivakumarBurnett School of Biomedical Sciences, UCF College of Medicine, University of Central Florida, Orlando, FL USA.
Mingyu ChaDepartment of Computer Science, University of Central Florida, Florida, USA.
Mariana Bernardo FiadeiroBurnett School of Biomedical Sciences, UCF College of Medicine, University of Central Florida, Orlando, FL USA.
Haiyan Nancy HuDepartment of Computer Science, University of Central Florida, Florida, USA.
Yoon-Seong KimBurnett School of Biomedical Sciences, UCF College of Medicine, University of Central Florida, Orlando, FL USA.

Funding

Contribution of transcriptional mutagenesis of oxidative DNA lesions to generatingnew mutant alpha-synuclein species and aggregation toward the pathogenesis of Parkinson'sdiseaseR01NS100919 · NINDS · UNIVERSITY OF CENTRAL FLORIDA · PI KIM, YOON-SEONG · 2018 to 2022
$1.7M
Pum2-dependent translational regulation of a-SYN near mitochondria and contribution to the pathogenesis of Parkinson's diseaseR01NS101461 · NINDS · UNIVERSITY OF CENTRAL FLORIDA · PI KIM, YOON-SEONG · 2018 to 2022
$1.7M
NINDS NIH HHS R01 NS100919NINDS NIH HHS R01 NS101461
6 · The paper itself

Abstract

Epigenetic regulation is a crucial factor controlling gene expression. Here, we report our CRISPR/dCas9-based modular epigenetic toolkit that enables gene-specific modulation of epigenetic architecture. By modifying the SunTag framework of dCas9 tagged with five GCN4 moieties, each epigenetic writer is bound to scFv and target-specific sgRNA, and this system is able to modify multiple epigenetic marks in a target-specific manner. We successfully demonstrated that this system is efficient in modifying individual histone post-translational modifications. We display its utility as a tool to understand the contributions of specific histone marks on gene expression by screening a large promoter region and identifying differential outcomes with high base-pair resolution. This epigenetic toolkit can be easily altered with a large variety of epigenetic effectors and is a useful tool for researchers to use in understanding gene-specific epigenetic changes and their relation to gene expression.

Indexed as

Epigenesis, GeneticEpigenomicsCRISPR-Cas SystemsDNA MethylationGene EditingPromoter Regions, GeneticCRISPREpigeneticsepigenetic writersepigenome editinghistone post-translational modifications

Identifiers

PMID35920441
PMCPMC9665145

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.