ArticleVeterinary research forum : an international quarterly journal2022
Preparation of reductive polypeptides from fresh placentas of dairy cows.
Article in Veterinary research forum : an international quarterly journal, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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3 citing papers in PubMed, 8 citations in OpenAlex.
- Preparation and characterization of renal cell peptides from fetal rats for their antitumor activity.FEBS open bio · 2025Article
- Analysis and Comparison of Proteomics of Placental Proteins from Cows Using Different Proteases.Animals : an open access journal from MDPI · 2023Article
- Antioxidant Capacity and Protective Effect of Cow Placenta Extract on D-Galactose-Induced Skin Aging in Mice.Nutrients · 2022Article
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Authors and funding
11 authors at 1 institution in 1 country.
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Abstract
In order to prepare reductive polypeptides from the placenta of dairy cows' fresh placentas from healthy Chinese Holstein cows were obtained and homogenized. Response surface model was established to optimize the hydrolysis condition for the extraction of the placental polypeptides. Specifically, the placental tissue homogenate was treated with both trypsin and pepsin for 348 min and 329 min; at 35.00% and 35.75% of substrate concentration; with an enzyme-substrate ratio of 3.33% and 3.92%, respectively, based on the models. The treated samples were then demineralized and freeze-dried to obtain the hydrolyzed polypeptides. In order to identify the molecular mass distribution and reducibility of polypeptides, matrix-assisted laser desorption ionization (MALDI) and Prussian blue methods were used. The concentrations of placental polypeptides after hydrolysis by trypsin or pepsin were 5.52% and 5.97%, respectively; the vitamin C (Vit C) equivalents were 36.26 μg mg
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