ArticleScientific reports2022
Modifying the yeast very long chain fatty acid biosynthetic machinery by the expression of plant 3-ketoacyl CoA synthase isozymes.
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed, 18 citations in OpenAlex.
- Molecular basis of very-long-chain fatty acid elongation by the CER6-GL2 enzyme complex in plant wax biosynthesis.Science advances · 2025Article
- Genome-wide analysis of KCS family in Medicago reveals MsKCS5's crucial role in abiotic stress adaptation.Plant cell reports · 2025Article
- Genome-Wide Analysis ofGenes · 2025Article
- In silico characterization of Rhodotorula toruloides ELO-like elongases and production of very-long-chain fatty acids by expressing Rtelo2, RtKCR, RtHCD, and RtECR through IRES-mediated bicistrons.World journal of microbiology & biotechnology · 2024Article
- Integration of Transcriptome and Metabolome Reveals Wax Serves a Key Role in Preventing Leaf Water Loss in Goji (International journal of molecular sciences · 2024Article
- Dynamic relationships among pathways producing hydrocarbons and fatty acids of maize silk cuticular waxes.Plant physiology · 2024Article
- GWAS and WGCNA Analysis Uncover Candidate Genes Associated with Oil Content in Soybean.Plants (Basel, Switzerland) · 2024Article
- The impact of the GLOSSY2 and GLOSSY2-LIKE BAHD-proteins in affecting the product profile of the maize fatty acid elongase.Frontiers in plant science · 2024Article
- Tackling functional redundancy of Arabidopsis fatty acid elongase complexes.Frontiers in plant science · 2023Article
- Genome-wide identification and expression analysis of 3-ketoacyl-CoA synthase gene family in rice (Frontiers in plant science · 2023Article
- Single-cell genetic models to evaluate orphan gene function: The case of QQS regulating carbon and nitrogen allocation.Frontiers in plant science · 2023Article
- Genetic dissection of QTLs for oil content in four maize DH populations.Frontiers in plant science · 2023Article
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5 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Eukaryotes express a multi-component fatty acid elongase to produce very long chain fatty acids (VLCFAs), which are building blocks of diverse lipids. Elongation is achieved by cyclical iteration of four reactions, the first of which generates a new carbon-carbon bond, elongating the acyl-chain. This reaction is catalyzed by either ELONGATION DEFECTIVE LIKE (ELO) or 3-ketoacyl-CoA synthase (KCS) enzymes. Whereas plants express both ELO and KCS enzymes, other eukaryotes express only ELOs. We explored the Zea mays KCS enzymatic redundancies by expressing each of the 26 isozymes in yeast strains that lacked endogenous ELO isozymes. Expression of the 26 maize KCS isozymes in wild-type, scelo2 or scelo3 single mutants did not affect VLCFA profiles. However, a complementation screen of each of the 26 KCS isozymes revealed five that were capable of complementing the synthetically lethal scelo2; scelo3 double mutant. These rescued strains express novel VLCFA profiles reflecting the different catalytic capabilities of the KCS isozymes. These novel strains offer a platform to explore the relationship between VLCFA profiles and cellular physiology.
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