ArticleNature methods2022
Photoswitching fingerprint analysis bypasses the 10-nm resolution barrier.
Article in Nature methods, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed.
- Single-Step Expansion SMLM Enables Molecular-Resolution Imaging in Cells and Isolated Proteins.Chemical & biomedical imaging · 2026Article
- Modulation of Single-Molecule Emission at Hexagonal Boron Nitride Surfaces.Nano letters · 2026Article
- Accelerated Left-Handed DNA-PAINT Using Fluorogenic Probes.Nano letters · 2026Article
- Energy transfer leaves fingerprints in cyanine photoswitching behavior.PLoS computational biology · 2026Article
- Impact of Docking Strand Design on Spatial Resolution in DNA-Points Accumulation for Imaging in Nanoscale Topography.Chemphyschem : a European journal of chemical physics and physical chemistry · 2026Article
- Molecular mapping in DNA-PAINT via modified Gaussian Mixture Modeling.Nature communications · 2026Article
- Labeling Postsynaptic Densities for Super-Resolution Microscopy With Minimal Signal-Loss and Offset.Bio-protocol · 2025Article
- Ångström-resolution imaging of cell-surface glycans.Nature nanotechnology · 2025Article
- One-step nanoscale expansion microscopy reveals individual protein shapes.Nature biotechnology · 2025Article
- Super-resolution microscopy for structural biology.Nature methods · 2025Review
- From Biophysics to Biomedical Physics.ACS bio & med chem Au · 2025Review
- A Bayesian Model to Count the Number of Two-State Emitters in a Diffraction Limited Spot.Nano letters · 2025Article
- Super-multiplexed imaging and coding in the range of radio frequency.Nature communications · 2025Article
- Fluorescent labeling strategies for molecular bioimaging.Biophysical reports · 2025Review
- Review
- The DNA-PAINT palette: a comprehensive performance analysis of fluorescent dyes.Nature methods · 2024Article
- Fast In Vitro Synthesis and Direct Labeling of Nanobodies for Prototyping in Microscopy Applications.ACS omega · 2024Article
- Effects and avoidance of photoconversion-induced artifacts in confocal and STED microscopy.Nature methods · 2024Article
- SEMORE: SEgmentation and MORphological fingErprinting by machine learning automates super-resolution data analysis.Nature communications · 2024Article
- Bioorthogonal Reactions in Bioimaging.Topics in current chemistry (Cham) · 2024Review
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Advances in super-resolution microscopy have demonstrated single-molecule localization precisions of a few nanometers. However, translation of such high localization precisions into sub-10-nm spatial resolution in biological samples remains challenging. Here we show that resonance energy transfer between fluorophores separated by less than 10 nm results in accelerated fluorescence blinking and consequently lower localization probabilities impeding sub-10-nm fluorescence imaging. We demonstrate that time-resolved fluorescence detection in combination with photoswitching fingerprint analysis can be used to determine the number and distance even of spatially unresolvable fluorophores in the sub-10-nm range. In combination with genetic code expansion with unnatural amino acids and bioorthogonal click labeling with small fluorophores, photoswitching fingerprint analysis can be used advantageously to reveal information about the number of fluorophores present and their distances in the sub-10-nm range in cells.
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