ArticleJournal of the American Chemical Society2022
Engineered Amber-Emitting Nano Luciferase and Its Use for Immunobioluminescence Imaging
Article in Journal of the American Chemical Society, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 39 citations in OpenAlex.
- A zebrafish luminescent biosensor for kidney tubulopathy, metal toxicity and drug screening.Disease models & mechanisms · 2026Article
- NirFAP680: a highly bright and stable large Stokes shift near-infrared fluorogen-activating protein.Nature methods · 2026Article
- Expanded applications of bioluminescence microscopy with phasor analysis.Cell reports methods · 2026Article
- Chemical biology approaches to study and target circadian clocks and their components.FEBS letters · 2026Review
- Development of a novel NanoBRET high-throughput drug screening assay for human GnRH receptor using sulfo-cyanine 5 fluorophore.Journal of pharmaceutical analysis · 2026Article
- Continuous Hypermutation and Evolution of Luciferase Variants.ACS chemical biology · 2026Article
- Protein engineering: status report.Protein engineering, design & selection : PEDS · 2026Review
- Tumor-targeting bioluminescent bacteria forEngineering microbiology · 2025Article
- Signal Transduction Strategies for DNAzyme-Based Sensing and Imaging of Metal Ions in Cells andChemical & biomedical imaging · 2025Review
- An Enhanced Red Bioluminescent Indicator for Responsive Detection of Physiological Calcium Dynamics in Cells and Mice.ACS sensors · 2025Article
- The latest developments of near-infrared fluorescent probes from NIR-I to NIR-II for bioimaging.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2025Review
- Article
- Coelenterazine Analogs for Bioassays and Molecular Imaging.Sensors (Basel, Switzerland) · 2025Review
- A Bioluminescent Probe for HACS bio & med chem Au · 2025Article
- PEGylated ATP-Independent Luciferins for Noninvasive High-Sensitivity High-Speed Bioluminescence Imaging.ACS chemical biology · 2025Article
- OptimizediScience · 2024Article
- Bright Red Bioluminescence from Semisynthetic NanoLuc (sNLuc).ACS chemical biology · 2024Article
- Bioluminescence Imaging of Potassium Ion Using a Sensory Luciferin and an Engineered Luciferase.Journal of the American Chemical Society · 2024Article
- Bioluminescence-Based Determination of Cytosolic Accumulation of Antibiotics inACS infectious diseases · 2024Article
- Recent advances in bioluminescent probes for neurobiology.Neurophotonics · 2024Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
The NanoLuc luciferase (NLuc) and its furimazine (FRZ) substrate have revolutionized bioluminescence (BL) assays and imaging. However, the use of the NLuc-FRZ luciferase-luciferin pair for mammalian tissue imaging is hindered by the low tissue penetration of the emitting blue photons. Here, we present the development of an NLuc mutant, QLuc, which catalyzes the oxidation of a synthetic QTZ luciferin for bright and red-shifted emission peaking at ∼585 nm. Compared to other small single-domain NLuc mutants, this amber-light-emitting luciferase exhibited improved performance for imaging deep-tissue targets in live mice. Leveraging this novel bioluminescent reporter, we further pursued
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