Evidence map›Paper›PMID 35913154›Full record

ArticleMicrobiology spectrum2022

LPS O Antigen Plays a Key Role in Klebsiella pneumoniae Capsule Retention.

Shweta Singh, Jonathan J Wilksch, Rhys A Dunstan, Anna Mularski, Nancy Wang, Dianna Hocking, Leila Jebeli, Hanwei Cao, Abigail Clements, Adam W J Jenney and 2 more

Open access · goldAbstract read
In one paragraph

Article in Microbiology spectrum, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.

0numbers the graph read from it
0cells of the map it votes in
39citing papers in PubMed
3.6field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

39 citing papers in PubMed, 51 citations in OpenAlex.

  1. Review
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  7. H-NS Regulates the Virulence ofMicroorganisms · 2026
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  8. <p>Molecular medicine reports · 2026
    Review
  9. Frontiers in microbiology · 2026
    Review
  10. Article
  11. Review
  12. Article
  13. Article
  14. Article
  15. O-antigen polysaccharides inMicrobiology and molecular biology reviews : MMBR · 2025
    Review
  16. mBio · 2025
    Article
  17. Article
  18. Article
  19. Review
  20. Probiotic Characterization ofVeterinary sciences · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 2 countries.

Shweta Singh *Department of Microbiology and Immunology, University of Melbournegrid.1008.9 at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia.
Jonathan J Wilksch *Department of Microbiology and Immunology, University of Melbournegrid.1008.9 at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia.
Rhys A DunstanInfection and Immunity Program, Biomedicine Discovery Institute and Department of Microbiology, Monash Universitygrid.1002.3, Clayton, Victoria, Australia.ORCID 0000-0002-7161-3993
Anna MularskiDepartment of Microbiology and Immunology, University of Melbournegrid.1008.9 at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia.
Nancy WangDepartment of Microbiology and Immunology, University of Melbournegrid.1008.9 at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia.
Dianna HockingDepartment of Microbiology and Immunology, University of Melbournegrid.1008.9 at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia.
Leila JebeliDepartment of Microbiology and Immunology, University of Melbournegrid.1008.9 at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia.
Hanwei CaoDepartment of Microbiology and Immunology, University of Melbournegrid.1008.9 at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia.
Abigail ClementsDepartment of Life Sciences, Imperial College London, London, United Kingdom.ORCID 0000-0003-2285-777X
Adam W J JenneyDepartment of Microbiology and Immunology, University of Melbournegrid.1008.9 at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia.
Trevor LithgowInfection and Immunity Program, Biomedicine Discovery Institute and Department of Microbiology, Monash Universitygrid.1002.3, Clayton, Victoria, Australia.ORCID 0000-0002-0102-7884
Richard A StrugnellDepartment of Microbiology and Immunology, University of Melbournegrid.1008.9 at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia.ORCID 0000-0003-0614-5641
The University of Melbourne · AUAustralian Regenerative Medicine Institute · AUImperial College London · GB

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Despite the importance of encapsulation in bacterial pathogenesis, the biochemical mechanisms and forces that underpin retention of capsule by encapsulated bacteria are poorly understood. In Gram-negative bacteria, there may be interactions between lipopolysaccharide (LPS) core and capsule polymers, between capsule polymers with retained acyl carriers and the outer membrane, and in some bacteria, between the capsule polymers and Wzi, an outer membrane protein lectin. Our transposon studies in Klebsiella pneumoniae B5055 identified additional genes that, when insertionally inactivated, resulted in reduced encapsulation. Inactivation of the gene

Indexed as

Klebsiella InfectionsKlebsiella pneumoniaeAnimalsBacterial CapsulesCapsulesHumansLipopolysaccharidesMiceO AntigensPolymersSugarsCapsulesLipopolysaccharidesO AntigensPolymersSugarscapsuleencapsulationKlebsiellaLPSO antigenretentionvirulence

Identifiers

PMID35913154
PMCPMC9431683
OpenAlexW4289238802

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.