ArticleBiochip journal2022
Inhibition of Non-specific Amplification in Loop-Mediated Isothermal Amplification via Tetramethylammonium Chloride.
Article in Biochip journal, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 49 citations in OpenAlex.
- Loop-mediated isothermal amplification-lateral flow dipstick (LAMP-LFD) for detection of wine microorganisms.World journal of microbiology & biotechnology · 2026Article
- Revolutionizing Healthcare With Paper-Based Nucleic Acid Testing.Exploration (Beijing, China) · 2026Review
- Nanomaterial-nucleic acid probe synergy: accelerating rapid pathogen detection and antimicrobial susceptibility testing in bloodstream infections.Folia microbiologica · 2026Review
- Suppressing Primer-Driven Nonspecific Amplification in LAMP Using TrueLAMP.bioRxiv : the preprint server for biology · 2026Article
- High-temperature ssBP-LAMP breaks the barrier of nonspecific amplification and unlocks the full potential of LAMP diagnostics.Cellular & molecular biology letters · 2026Article
- A Novel Loop-Mediated Isothermal Amplification (LAMP) Primer Set for Detecting the STY2879 Gene ofMicroorganisms · 2026Article
- Rapid subtype-specific detection of respiratory syncytial virus A and B using an RNase HII-dependent RT-LAMP lateral flow assay.Frontiers in chemistry · 2026Article
- Development of PCR, qPCR and LAMP methods for the detection ofFood and waterborne parasitology · 2025Article
- Next-Generation Nucleic Acid-Based Diagnostics for Viral Pathogens: Lessons Learned from the SARS-CoV-2 Pandemic.Microorganisms · 2025Review
- Toward Safer Biotherapeutics: Expression and Characterization of a Humanized Chimeric L-Asparaginase inInternational journal of molecular sciences · 2025Article
- Loop-Mediated Isothermal Amplification Assay for Visual Detection ofFoods (Basel, Switzerland) · 2025Article
- A one-pot method for universal Dengue virus detection by combining RT-RPA amplification and CRISPR/Cas12a assay.BMC microbiology · 2025Article
- Development of a Rapid Surveillance System for Ross River Virus in Mosquitoes Through Reverse-Transcription Loop-Mediated Isothermal Amplification (RT-LAMP).Transboundary and emerging diseases · 2025Article
- Innovative nucleic acid detection ofFrontiers in cellular and infection microbiology · 2025Article
- Enhanced Specificity in Colorimetric LAMP Assay forJournal of fungi (Basel, Switzerland) · 2024Article
- Evaluating Commercial Loop-Mediated Isothermal Amplification Master Mixes for Enhanced Detection of Foodborne Pathogens.Foods (Basel, Switzerland) · 2024Article
- Dual-Mode Graphene Field-Effect Transistor Biosensor with Isothermal Nucleic Acid Amplification.Biosensors · 2024Article
- A reverse transcription loop-mediated isothermal amplification assay for quick detection of tomato mosaic virus.PloS one · 2024Article
- Metaverse Wearables for Immersive Digital Healthcare: A Review.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Review
- Loop-mediated isothermal amplification assay for screening congenital cytomegalovirus infection in newborns.Applied microbiology and biotechnology · 2023Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Loop-mediated isothermal amplification (LAMP) may be used in molecular and point-of-care diagnostics for pathogen detection. The amplification occurs under isothermal conditions using up to six primers. However, non-specific amplification is frequently observed in LAMP. Non-specific amplification has the potential to be triggered by forward and reverse internal primers. And the relatively low reaction temperature (55-65 °C) induces the secondary structure via primer-primer interactions. Primer redesign and probe design have been recommended to solve this problem. LAMP primers have strict conditions, such as Tm, GC contents, primer dimer, and distance between primers compared to conventional PCR primers. Probe design requires specialized knowledge to have high specificity for a target. In polymerase chain reaction (PCR), some chemicals or proteins are used for improving specificity and efficiency. Therefore, we hypothesized that additives can suppress the non-specific amplification. In this study, tetramethylammonium chloride (TMAC), formamide, dimethyl sulfoxide, Tween 20, and bovine serum albumin have been used as LAMP additives. In our study, TMAC was presented as a promising additive for suppressing non-specific amplification in LAMP. Supplementary Information: The online version contains supplementary material available at 10.1007/s13206-022-00070-3.
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