Epidemiological surveillance of Newcastle disease virus in Egypt - a 6-year cohort study.

Newcastle disease (ND) is one of the most important poultry diseases worldwide and can lead to annual losses of up to 80% of backyard chickens in Africa. A retrospective cohort of 6 years was planned to screen the NDV in intensive chicken and turkey flocks. The existence of velogenic NDV strain was screened in different poultry flocks showing suspected signs of NDV using real-time RT-PCR targeting the F gene of the velogenic strain. A total of 843 poultry flocks were screened during the cohort. Samples were classified based on the month and year as well as the poultry type. All flocks should be negative for avian influenza virus as an inclusion criterion of the study. The F gene of a randomly selected positive sample from each year as well as an archival sample from 2005 was sequenced. An overall of 52.4% (443/842) of the tested farms showed positive results for the velogenic NDV. The cumulative percentage of positive flocks to the total positive flocks per month ranged from 5.9 to 11.8%. The results revealed that NDV is circulating across all months annually without evidence of seasonal tendency of the disease. Most of the strains belong to genotype VII.1.1, with only two strains related to XXI.1.1 and XXI.2. All VII.1.1 strains possess arginine at 27 position while XXI.1.1 and XXI.2 strains showed cysteine at 27 and amino acid substitutions in the signal peptide, cleavage site, and neutralizing epitopes. In conclusion, the current molecular epidemiological surveillance confirms the enzootic nature of NDV. It circulates all year round with no evidence of seasonal incidence. Genotype VII is the most predominant NDV genotype in Egypt.