Evidence map›Paper›PMID 35908795›Full record

ArticleJournal of clinical laboratory analysis2022

Assessment of recombinant antigens Tp0100 and Tp1016 of Treponema pallidum for serological diagnosis of syphilis.

Dejun Chen, Siqian Wang, Yuxing He, Yue Fu, Feijun Zhao, Xiuping Zhou, Haoquan Yin, Jia Wan, Yunting Huang, Yimou Wu and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of clinical laboratory analysis, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.8field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Comprehensive Overview ofCurrent protein & peptide science · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 4 institutions in 1 country.

Dejun ChenInstitution of Pathogenic Biology and Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Hengyang Medical School, University of South China, Hengyang, China.
Siqian WangClinical laboratory of the First Pepole's Hospital of Changde City, Changde, China.
Yuxing HeInstitution of Pathogenic Biology and Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Hengyang Medical School, University of South China, Hengyang, China.
Yue FuInstitution of Pathogenic Biology and Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Hengyang Medical School, University of South China, Hengyang, China.
Feijun ZhaoInstitution of Pathogenic Biology and Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Hengyang Medical School, University of South China, Hengyang, China.ORCID https://orcid.org/0000-0001-9542-6896
Xiuping ZhouDepartment of Laboratory Medicine, Changsha Health Vocational College, Changsha, China.
Haoquan YinDepartment of Clinical Medical Undergraduates, Hengyang Medical School, University of South China, Hengyang, China.
Jia WanDepartment of Clinical Medical Undergraduates, Hengyang Medical School, University of South China, Hengyang, China.
Yunting HuangDepartment of Clinical Medical Undergraduates, Hengyang Medical School, University of South China, Hengyang, China.
Yimou WuInstitution of Pathogenic Biology and Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Hengyang Medical School, University of South China, Hengyang, China.
Longgu CaoCollege of Medical Imaging Laboratory and Rehabilitation, Xiangnan University, Chenzhou, China.
Tiebing ZengInstitution of Pathogenic Biology and Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Hengyang Medical School, University of South China, Hengyang, China.ORCID https://orcid.org/0000-0002-4075-1613
University of South China · CNChangsha University · CNThe First People's Hospital of Changde · CNXiangnan University · CN

Funding

Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study 2015-351Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control Foundation 2014-5Major Scientific and Technological Projects for Collaborative Prevention and Control of Birth Defects in Hunan Province 2019SK1010National Natural Science Foundation of China 81273322Research Learning and Innovative Experiment Project for College Students in Hunan Province S201910555134Research Learning and Innovative Experiment Project for College Students in Hunan Province S202010555056Research Learning and Innovative Experiment Project for College Students in Hunan Province S202110555306Scientific Research Fund of Hunan Provincial Education Department 20A424Scientific Research Fund of Hunan Provincial Education Department 20C1732Scientific Research Fund of Hunan Provincial Education Department 21C1650
6 · The paper itself

Abstract

objectiveTo discover novel serodiagnostic candidates for the serological diagnosis of syphilis.

methodsTwo recombinant Treponema pallidum proteins Tp0100 and Tp1016 were expressed, purified, and identified by Western Blotting. A total of 600 clinical serum samples were tested with the Tp0100-based ELISA, the Tp1016-based ELISA, and the commercial LICA Syphilis TP kit (ChIVD, Beijing, China). The sensitivities were determined by testing 340 samples from individuals with clinically diagnosed primary, secondary, latent, and tertiary syphilis. The specificities were determined by screening 260 samples from healthy controls and individuals with potentially cross-reactive infections, including leptospirosis, Lyme disease, hepatitis B, tuberculosis, rheumatoid arthritis, systemic lupus erythematosus. Kappa (κ) values were applied to compare the agreement between clinical syphilis diagnosis and the Tp0100-based ELISA, the Tp1016-based ELISA, or the LICA Syphilis TP test.

resultsUsing clinical syphilis diagnosis as the gold standard, Tp0100 exhibited an overall sensitivity of 95.6% and specificity of 98.1% for testing IgG antibody while Tp1016 demonstrated only an overall sensitivity of 75.0% and specificity of 79.6%. In contrast, the LICA Syphilis TP test revealed an overall sensitivity of 97.6% and specificity of 96.2%. In addition, the overall percent agreement and corresponding κ values were 96.7% (95% CI 95.6%-97.8%) and 0.93 for the Tp0100-based ELISA, 77.0% (95% CI 74.3%-79.7%) and 0.54 for the Tp1016-based ELISA, and 97.0% (95% CI 96.0%-98.0%) and 0.94 for the LICA Syphilis TP test, respectively.

conclusionThe recombinant T. pallidum protein Tp0100 shows promise as a novel diagnostic antigen in the serological tests for syphilis.

Indexed as

SyphilisTreponema pallidumAntibodies, BacterialAntigens, BacterialEnzyme-Linked Immunosorbent AssayHumansRecombinant ProteinsSensitivity and SpecificitySerologic TestsSyphilis SerodiagnosisAntibodies, BacterialAntigens, BacterialRecombinant ProteinsELISArecombinant proteinserodiagnosissyphilisTp0100Tp1016Treponema pallidum

Identifiers

PMID35908795
PMCPMC9459255
OpenAlexW4289101561

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.