ArticleVirology journal2022
Reverse transcription recombinase-aided amplification assay for H5 subtype avian influenza virus.
Article in Virology journal, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 11 citations in OpenAlex.
- Development of a recombinase-aided amplification assay for rapid and sensitive detection ofExperimental and therapeutic medicine · 2026Article
- Recent advances in isothermal amplification techniques for detection of animal diseases.Frontiers in veterinary science · 2026Review
- Establishment and comparison of RF-RAA and qPCR for rapid detection of Chinese soft-shelled turtle adenovirus (CSTAdV).Virology journal · 2025Article
- A New Dual Fluorescence Method for Rapid Detection of Infectious Bronchitis Virus at Constant Temperature.Microorganisms · 2024Article
- Dual Gene Detection of H5N1 Avian Influenza Virus Based on Dual RT-RPA.Molecules (Basel, Switzerland) · 2024Article
- Advances in Detection Techniques for the H5N1 Avian Influenza Virus.International journal of molecular sciences · 2023Review
- Development of rapid nucleic acid assays based on the recombinant polymerase amplification for monkeypox virus.Virologica Sinica · 2023Article
- Based on CRISPR-Cas13a system, to establish a rapid visual detection method for avian influenza viruses.Frontiers in veterinary science · 2023Article
- Advancements in the synergy of isothermal amplification and CRISPR-cas technologies for pathogen detection.Frontiers in bioengineering and biotechnology · 2023Review
- Advances in the application of recombinase-aided amplification combined with CRISPR-Cas technology in quick detection of pathogenic microbes.Frontiers in bioengineering and biotechnology · 2023Review
- Rapid detection of influenza A viruses using a real-time reverse transcription recombinase-aided amplification assay.Frontiers in cellular and infection microbiology · 2022Article
Corrections and comments
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Authors and funding
14 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe H5 subtype avian influenza virus (AIV) has caused huge economic losses to the poultry industry and is a threat to human health. A rapid and simple test is needed to confirm infection in suspected cases during disease outbreaks.
methodsIn this study, we developed a reverse transcription recombinase-aided amplification (RT-RAA) assay for the detection of H5 subtype AIV. Assays were performed at a single temperature (39 °C), and the results were obtained within 20 min.
resultsThe assay showed no cross-detection with Newcastle disease virus or infectious bronchitis virus. The analytical sensitivity was 10
conclusionsThese results indicated that our RT-RAA assay may be a valuable tool for detecting H5 subtype AIV.
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Registered trials
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