ArticleEuropean biophysics journal : EBJ2022
Neurotransmitter uptake of synaptic vesicles studied by X-ray diffraction.
Article in European biophysics journal : EBJ, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 6 citations in OpenAlex.
- The European Biophysics Journal Prizes 2025: Recognising biophysical science at sub-cellular, cellular and tissue levels of organisation.European biophysics journal : EBJ · 2026Review
- "Kiss-shrink-run" unifies mechanisms for synaptic vesicle exocytosis and hyperfast recycling.Science (New York, N.Y.) · 2025Article
- Synaptophysin accelerates synaptic vesicle fusion by expanding the membrane upon neurotransmitter loading.Science advances · 2025Article
- Morphology and intervesicle distances in condensates of synaptic vesicles and synapsin.Biophysical journal · 2024Article
- Vesicle condensation induced by synapsin: condensate size, geometry, and vesicle shape deformations.The European physical journal. E, Soft matter · 2024Article
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
The size, polydispersity, and electron density profile of synaptic vesicles (SVs) can be studied by small-angle X-ray scattering (SAXS), i.e. by X-ray diffraction from purified SV suspensions in solution. Here we show that size and shape transformations, as they appear in the functional context of these important synaptic organelles, can also be monitored by SAXS. In particular, we have investigated the active uptake of neurotransmitters, and find a mean vesicle radius increase of about 12% after the uptake of glutamate, which indicates an unusually large extensibility of the vesicle surface, likely to be accompanied by conformational changes of membrane proteins and rearrangements of the bilayer. Changes in the electron density profile (EDP) give first indications for such a rearrangement. Details of the protein structure are screened, however, by SVs polydispersity. To overcome the limitations of large ensemble averages and heterogeneous structures, we therefore propose serial X-ray diffraction by single free electron laser pulses. Using simulated data for realistic parameters, we show that this is in principle feasible, and that even spatial distances between vesicle proteins could be assessed by this approach.
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Registered trials
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