ArticleNature communications2022
De novo determination of mosquitocidal Cry11Aa and Cry11Ba structures from naturally-occurring nanocrystals.
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Article in Nature communications, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13citing papers in PubMed
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13 citing papers in PubMed.
- Interaction with theApplied and environmental microbiology · 2026Article
- Electron fourier ptychography for phase reconstruction.Scientific reports · 2025Article
- Intracellular protein crystallization in living insect cells.FEBS open bio · 2025Article
- Essential Role of Non-Conserved α4-HisProtein and peptide letters · 2025Article
- A snapshot love story: what serial crystallography has done and will do for us.Acta crystallographica. Section D, Structural biology · 2024Review
- Review
- Review
- A streamlined approach to structure elucidation using in cellulo crystallized recombinant proteins, InCellCryst.Nature communications · 2024Article
- Mutational analysis of the transmembrane α4-helix of Bacillus thuringiensis mosquito-larvicidal Cry4Aa toxin.Current microbiology · 2024Article
- Structure of theProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Channel Formation in Cry Toxins: An Alphafold-2 Perspective.International journal of molecular sciences · 2023Article
- Toxic Determination of Cry11 Mutated Proteins Obtained Using Rational Design and Its Computational Analysis.International journal of molecular sciences · 2023Article
- Structural changes upon membrane insertion of the insecticidal pore-forming toxins produced byFrontiers in insect science · 2023Review
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Authors and funding
63 authors.
Guillaume Tetreau *Univ. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale, 71 Avenue des martyrs, F-38000, Grenoble, France.
Michael R Sawaya *UCLA-DOE Institute for Genomics and Proteomics, Department of Biological Chemistry, University of California, Los Angeles, CA, 90095-1570, USA.ORCID 0000-0003-0874-9043
Elke De Zitter *Univ. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale, 71 Avenue des martyrs, F-38000, Grenoble, France.ORCID 0000-0001-6325-7354
Elena A AndreevaUniv. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale, 71 Avenue des martyrs, F-38000, Grenoble, France.ORCID 0000-0002-2931-3698
Anne-Sophie BannevilleUniv. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale, 71 Avenue des martyrs, F-38000, Grenoble, France.ORCID 0000-0002-0698-6291
Natalie A SchibrowskyUCLA-DOE Institute for Genomics and Proteomics, Department of Biological Chemistry, University of California, Los Angeles, CA, 90095-1570, USA.
Nicolas CoquelleLarge-Scale Structures Group, Institut Laue-Langevin, F-38000, Grenoble, France.
Aaron S BrewsterMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.ORCID 0000-0002-0908-7822
Marie Luise GrünbeinMax-Planck-Institut für medizinische Forschung, Jahnstrasse 29, 69120, Heidelberg, Germany.
Gabriela Nass KovacsMax-Planck-Institut für medizinische Forschung, Jahnstrasse 29, 69120, Heidelberg, Germany.
Mark S HunterLinac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA, 94025, USA.ORCID 0000-0002-0110-7075
Marco KloosMax-Planck-Institut für medizinische Forschung, Jahnstrasse 29, 69120, Heidelberg, Germany.
Raymond G SierraLinac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA, 94025, USA.ORCID 0000-0002-6253-8282
Giorgio SchiroUniv. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale, 71 Avenue des martyrs, F-38000, Grenoble, France.
Pei QiaoDepartment of Chemistry, Texas A&M University, College Station, TX, 77845, USA.
Myriam StrickerMax-Planck-Institut für medizinische Forschung, Jahnstrasse 29, 69120, Heidelberg, Germany.ORCID 0000-0002-3397-2418
Dennis BideshiDepartment of Entomology and Institute for Integrative Genome Biology, University of California, Riverside, CA, 92521, USA.
Iris D YoungMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.ORCID 0000-0003-4713-9504
Ninon ZalaUniv. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale, 71 Avenue des martyrs, F-38000, Grenoble, France.
Sylvain EngilbergeUniv. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale, 71 Avenue des martyrs, F-38000, Grenoble, France.
Alexander GorelMax-Planck-Institut für medizinische Forschung, Jahnstrasse 29, 69120, Heidelberg, Germany.
Luca SignorUniv. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale, 71 Avenue des martyrs, F-38000, Grenoble, France.ORCID 0000-0003-3880-2466
Jean-Marie TeulonUniv. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale, 71 Avenue des martyrs, F-38000, Grenoble, France.
Mario HilpertMax-Planck-Institut für medizinische Forschung, Jahnstrasse 29, 69120, Heidelberg, Germany.
Lutz FoucarMax-Planck-Institut für medizinische Forschung, Jahnstrasse 29, 69120, Heidelberg, Germany.
Johan BieleckiEuropean XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.
Richard BeanEuropean XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.
Raphael de WijnEuropean XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.
Tokushi SatoEuropean XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.ORCID 0000-0003-3155-3487
Henry KirkwoodEuropean XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.ORCID 0000-0001-6792-3844
Romain LetrunEuropean XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.ORCID 0000-0002-0569-5193
Alexander BatyukLinac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA, 94025, USA.ORCID 0000-0002-9393-2880
Irina SnigirevaEuropean Synchrotron Radiation Facility (ESRF), BP 220, 38043, Grenoble, France.ORCID 0000-0003-3481-2202
Daphna FenelUniv. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale, 71 Avenue des martyrs, F-38000, Grenoble, France.
Robin SchubertEuropean XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.ORCID 0000-0002-6213-2872
Ethan J CanfieldMass Spectrometry Core Facility, School of Pharmacy, University of Southern California, Los Angeles, CA, 90089, USA.
Mario M AlbaDepartment of Pharmacology and Pharmaceutical Sciences, School of Pharmacy, University of Southern California, Los Angeles, CA, 90089, USA.ORCID 0000-0002-0587-8790
Frédéric LaporteUniv. Grenoble Alpes, CNRS, LECA, F-38000, Grenoble, France.ORCID 0000-0002-5638-4328
Laurence DesprésUniv. Grenoble Alpes, CNRS, LECA, F-38000, Grenoble, France.ORCID 0000-0002-0660-6260
Maria BaciaUniv. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale, 71 Avenue des martyrs, F-38000, Grenoble, France.
Amandine RouxUniv. Lyon, ENS de Lyon, CNRS UMR 5182, Université Claude Bernard Lyon 1, Laboratoire de Chimie, F-69342, Lyon, France.
Christian ChapellePolyvalan SARL, 15 parvis René Descartes, 69342, Lyon, France.
François RiobéUniv. Lyon, ENS de Lyon, CNRS UMR 5182, Université Claude Bernard Lyon 1, Laboratoire de Chimie, F-69342, Lyon, France.ORCID 0000-0001-6746-8132
Olivier MauryUniv. Lyon, ENS de Lyon, CNRS UMR 5182, Université Claude Bernard Lyon 1, Laboratoire de Chimie, F-69342, Lyon, France.
Wai Li LingUniv. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale, 71 Avenue des martyrs, F-38000, Grenoble, France.ORCID 0000-0002-4264-5750
Sébastien BoutetLinac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA, 94025, USA.ORCID 0000-0003-3928-1244
Adrian MancusoEuropean XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.
Irina GutscheUniv. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale, 71 Avenue des martyrs, F-38000, Grenoble, France.ORCID 0000-0002-1908-3921
Eric GirardUniv. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale, 71 Avenue des martyrs, F-38000, Grenoble, France.ORCID 0000-0002-5758-6095
Thomas R M BarendsMax-Planck-Institut für medizinische Forschung, Jahnstrasse 29, 69120, Heidelberg, Germany.ORCID 0000-0002-9488-3005
Jean-Luc PellequerUniv. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale, 71 Avenue des martyrs, F-38000, Grenoble, France.ORCID 0000-0002-8944-2715
Hyun-Woo ParkDepartment of Entomology and Institute for Integrative Genome Biology, University of California, Riverside, CA, 92521, USA.
Arthur D LaganowskyDepartment of Chemistry, Texas A&M University, College Station, TX, 77845, USA.
Jose RodriguezUCLA-DOE Institute for Genomics and Proteomics, Department of Biological Chemistry, University of California, Los Angeles, CA, 90095-1570, USA.
Manfred BurghammerEuropean Synchrotron Radiation Facility (ESRF), BP 220, 38043, Grenoble, France.
Robert L ShoemanMax-Planck-Institut für medizinische Forschung, Jahnstrasse 29, 69120, Heidelberg, Germany.ORCID 0000-0002-1030-6738
R Bruce DoakMax-Planck-Institut für medizinische Forschung, Jahnstrasse 29, 69120, Heidelberg, Germany.
Martin WeikUniv. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale, 71 Avenue des martyrs, F-38000, Grenoble, France.ORCID 0000-0001-9297-642X
Nicholas K SauterMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.ORCID 0000-0003-2786-6552
Brian FedericiDepartment of Entomology and Institute for Integrative Genome Biology, University of California, Riverside, CA, 92521, USA.
Duilio CascioUCLA-DOE Institute for Genomics and Proteomics, Department of Biological Chemistry, University of California, Los Angeles, CA, 90095-1570, USA.ORCID 0000-0001-8285-754X
Ilme SchlichtingMax-Planck-Institut für medizinische Forschung, Jahnstrasse 29, 69120, Heidelberg, Germany.ORCID 0000-0002-0936-7496
Jacques-Philippe ColletierUniv. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale, 71 Avenue des martyrs, F-38000, Grenoble, France. colletier@ibs.fr.ORCID 0000-0003-1819-4686
Funding
ZWITTERION SURFACTANT DDMAB FOR IMPROV STABIL OF PROTEINP41RR001209 · NCRR · STANFORD UNIVERSITY · PI GONZALEZ, ANA M · 1985 to 2011
$38.9MX-ray Absorption Spectroscopy (XAS) pp.711-759P41GM103393 · NIGMS · STANFORD UNIVERSITY · PI HODGSON, KEITH O · 2012 to 2019
$30.6MUCLA NIGMS T32 Program AssessmentT32GM007185 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI TORRES, JORGE · 1985 to 2020
$21.7MDIALS: New Computational Methods to Enable Challenging Crystallographic ExperimentsR01GM117126 · NIGMS · UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB · PI SAUTER, NICHOLAS K · 2016 to 2023
$6.1MACL HHS U01CK000516NCEZID CDC HHS U01 CK000516NCRR NIH HHS P41 RR001209NIGMS NIH HHS P41 GM103393NIGMS NIH HHS R01 GM117126NIGMS NIH HHS T32 GM007185
Abstract
Cry11Aa and Cry11Ba are the two most potent toxins produced by mosquitocidal Bacillus thuringiensis subsp. israelensis and jegathesan, respectively. The toxins naturally crystallize within the host; however, the crystals are too small for structure determination at synchrotron sources. Therefore, we applied serial femtosecond crystallography at X-ray free electron lasers to in vivo-grown nanocrystals of these toxins. The structure of Cry11Aa was determined de novo using the single-wavelength anomalous dispersion method, which in turn enabled the determination of the Cry11Ba structure by molecular replacement. The two structures reveal a new pattern for in vivo crystallization of Cry toxins, whereby each of their three domains packs with a symmetrically identical domain, and a cleavable crystal packing motif is located within the protoxin rather than at the termini. The diversity of in vivo crystallization patterns suggests explanations for their varied levels of toxicity and rational approaches to improve these toxins for mosquito control.
Indexed as
Bacillus thuringiensisNanoparticlesAnimalsBacterial ProteinsEndotoxinsHemolysin ProteinsLarvaMosquito ControlBacterial ProteinsEndotoxinsHemolysin Proteins
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