Evidence map›Paper›PMID 35901078›Full record

ArticleACS synthetic biology2022

Building the Plant SynBio Toolbox through Combinatorial Analysis of DNA Regulatory Elements.

Alexander C Pfotenhauer, Alessandro Occhialini, Mary-Anne Nguyen, Helen Scott, Lezlee T Dice, Stacee A Harbison, Li Li, D Nikki Reuter, Tayler M Schimel, C Neal Stewart and 2 more

Open access · bronzeAbstract read
In one paragraph

Article in ACS synthetic biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
1.6field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 21 citations in OpenAlex.

  1. Review
  2. Article
  3. Design and Testing of Root-Specific Synthetic Promoters by Machine Learning.International journal of molecular sciences · 2026
    Article
  4. Review
  5. Review
  6. Review
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 2 institutions in 1 country.

Alexander C PfotenhauerDepartment of Food Science, University of Tennessee Knoxville, 102 Food Safety and Processing Building 2600 River Dr., Knoxville, Tennessee 37996, United States.
Alessandro OcchialiniDepartment of Food Science, University of Tennessee Knoxville, 102 Food Safety and Processing Building 2600 River Dr., Knoxville, Tennessee 37996, United States.
Mary-Anne NguyenDepartment of Food Science, University of Tennessee Knoxville, 102 Food Safety and Processing Building 2600 River Dr., Knoxville, Tennessee 37996, United States.
Helen ScottIntelligent Software and Systems, Raytheon BBN Technologies, Cambridge, Massachusetts 02138, United States.
Lezlee T DiceDepartment of Food Science, University of Tennessee Knoxville, 102 Food Safety and Processing Building 2600 River Dr., Knoxville, Tennessee 37996, United States.
Stacee A HarbisonDepartment of Food Science, University of Tennessee Knoxville, 102 Food Safety and Processing Building 2600 River Dr., Knoxville, Tennessee 37996, United States.
Li LiDepartment of Food Science, University of Tennessee Knoxville, 102 Food Safety and Processing Building 2600 River Dr., Knoxville, Tennessee 37996, United States.
D Nikki ReuterDepartment of Food Science, University of Tennessee Knoxville, 102 Food Safety and Processing Building 2600 River Dr., Knoxville, Tennessee 37996, United States.
Tayler M SchimelDepartment of Food Science, University of Tennessee Knoxville, 102 Food Safety and Processing Building 2600 River Dr., Knoxville, Tennessee 37996, United States.
C Neal StewartCenter for Agricultural Synthetic Biology, University of Tennessee Institute of Agriculture, Knoxville, Tennessee 37996, United States.
Jacob BealIntelligent Software and Systems, Raytheon BBN Technologies, Cambridge, Massachusetts 02138, United States.
Scott C LenaghanDepartment of Food Science, University of Tennessee Knoxville, 102 Food Safety and Processing Building 2600 River Dr., Knoxville, Tennessee 37996, United States.ORCID 0000-0002-7539-1726
University of Tennessee at Knoxville · USRTX (United States) · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

While the installation of complex genetic circuits in microorganisms is relatively routine, the synthetic biology toolbox is severely limited in plants. Of particular concern is the absence of combinatorial analysis of regulatory elements, the long design-build-test cycles associated with transgenic plant analysis, and a lack of naming standardization for cloning parts. Here, we use previously described plant regulatory elements to design, build, and test 91 transgene cassettes for relative expression strength. Constructs were transiently transfected into

Indexed as

NicotianaSynthetic BiologyDNAPlant LeavesPlants, Genetically ModifiedDNAflow cytometryfluorometrygenetic regulatory elementssingle-cell analysissynthetic biologytransgene expression

Identifiers

PMID35901078
PMCPMC9396662
OpenAlexW4288425568

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.