ArticleG3 (Bethesda, Md.)2022
Modular safe-harbor transgene insertion for targeted single-copy and extrachromosomal array integration in Caenorhabditis elegans.
Article in G3 (Bethesda, Md.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
37 citing papers in PubMed, 50 citations in OpenAlex.
- Targeted integration of extrachromosomal arrays inScience advances · 2026Article
- FLInt 2.0: robust and customizable single-shot integration in C. elegans.G3 (Bethesda, Md.) · 2026Article
- Endogenous autophagosome reporters reveal redundant and non-redundant roles of LGG-1 and LGG-2 inbioRxiv : the preprint server for biology · 2026Article
- sept-1/zina-1 is an ancient toxin-antidote system in Caenorhabditis elegans.PLoS biology · 2026Article
- Large transgene arrays cause aberrant transcription and synthetic molting defects withbioRxiv : the preprint server for biology · 2026Article
- Tunable tau expression in C. elegans neurons reveals that early-AD tau phosphorylation selectively impacts behavior and mitochondrial quality control.Neurobiology of disease · 2026Article
- The Caenorhabditis Genetics Center Curated Special Collections: a guide to protein degradation systems.Genetics · 2026Review
- Modular gene tagging inResearch square · 2026Article
- Modular gene tagging inbioRxiv : the preprint server for biology · 2026Article
- FLInt 2.0: Robust and customizable single shot integration inbioRxiv : the preprint server for biology · 2026Article
- Transgenesis and targeted mutagenesis in the human-parasitic nematodeFrontiers in parasitology · 2026Review
- Tau clearance reverses touch neuron dysfunction in both young and agedJournal of Alzheimer's disease : JAD · 2026Article
- Engineering the auxin-inducible degron system for tunable in vivo control of organismal physiology.Nature communications · 2025Article
- High-efficiency targeted integration of extrachromosomal arrays inbioRxiv : the preprint server for biology · 2025Article
- A single-copy knock-in system: one plasmid to target all chromosomes in C. elegans.G3 (Bethesda, Md.) · 2025Article
- Axon termination of the SAB motor neurons inbioRxiv : the preprint server for biology · 2025Article
- Regulation of MORC-1 is key to the CSR-1-mediated germline gene licensing mechanism inScience advances · 2025Article
- Intraspecific variation in the duration of epigenetic inheritance.bioRxiv : the preprint server for biology · 2025Article
- Parameters that influence bipartite reporter system expression in Caenorhabditis elegans.Genetics · 2025Article
- Multiscale patterning of a model apical extracellular matrix revealed by systematic endogenous protein tagging.bioRxiv : the preprint server for biology · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Efficient and reproducible transgenesis facilitates and accelerates research using genetic model organisms. Here, we describe a modular safe-harbor transgene insertion (MosTI) for use in Caenorhabditis elegans which improves targeted insertion of single-copy transgenes by homology directed repair and targeted integration of extrachromosomal arrays by nonhomologous end-joining. MosTI allows easy conversion between selection markers at insertion site and a collection of universal targeting vectors with commonly used promoters and fluorophores. Insertions are targeted at three permissive safe-harbor intergenic locations and transgenes are reproducibly expressed in somatic and germ cells. Chromosomal integration is mediated by CRISPR/Cas9, and positive selection is based on a set of split markers (unc-119, hygroR, and gfp) where only animals with chromosomal insertions are rescued, resistant to antibiotics, or fluorescent, respectively. Single-copy insertion is efficient using either constitutive or heat-shock inducible Cas9 expression (25-75%) and insertions can be generated from a multiplexed injection mix. Extrachromosomal array integration is also efficient (7-44%) at modular safe-harbor transgene insertion landing sites or at the endogenous unc-119 locus. We use short-read sequencing to estimate the plasmid copy numbers for 8 integrated arrays (6-37 copies) and long-read Nanopore sequencing to determine the structure and size (5.4 Mb) of 1 array. Using universal targeting vectors, standardized insertion strains, and optimized protocols, it is possible to construct complex transgenic strains which should facilitate the study of increasingly complex biological problems in C. elegans.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.