Evidence map›Paper›PMID 35899934›Full record

ArticleCell cycle (Georgetown, Tex.)2022

Effects of Cyclosporine A and Adalimumab on the expression profiles histaminergic system-associated genes and microRNAs regulating these genes in HaCaT cells.

Tomasz Kasela, Maciej Dąbala, Magdalena Mistarz, Weronika Wieczorek, Magdalena Wierzbik-Strońska, Kacper Boroń, Barbara Zawidlak-Węgrzyńska, Beniamin Oskar Grabarek

Open access · hybridAbstract read
In one paragraph

Article in Cell cycle (Georgetown, Tex.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.8field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Tomasz KaselaDepartment of Dermatology, European Center of Aesthetics in Katowice, Katowice, Poland.
Maciej DąbalaFaculty of Medicine, Dabala Ortodoncja in Katowice, Katowice, Poland.
Magdalena MistarzDepartment of Histology, Cytophysiology, and Embryology in Zabrze, Faculty of Medicine in Zabrze, The University of Technology, Academy of Silesia in Katowice, Zabrze, Poland.
Weronika WieczorekDepartment of Histology, Cytophysiology, and Embryology in Zabrze, Faculty of Medicine in Zabrze, The University of Technology, Academy of Silesia in Katowice, Zabrze, Poland.
Magdalena Wierzbik-StrońskaFaculty of Medicine in Zabrze, The University of Technology, Academy of Silesia in Katowice in Katowice, Zabrze, Poland.
Kacper BorońDepartment of Histology, Cytophysiology, and Embryology in Zabrze, Faculty of Medicine in Zabrze, The University of Technology, Academy of Silesia in Katowice, Zabrze, Poland.
Barbara Zawidlak-WęgrzyńskaFoundation for Cardiac Surgery Development, Heart Prosthesis Institute, Zabrze, Poland.
Beniamin Oskar GrabarekDepartment of Histology, Cytophysiology, and Embryology in Zabrze, Faculty of Medicine in Zabrze, The University of Technology, Academy of Silesia in Katowice, Zabrze, Poland.
Academy of Fine Arts in Katowice · PLFoundation of Cardiac Surgery Development · PLWSB University · PL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Previous studies have not completely elucidated the role of the histaminergic system in the pathogenesis of psoriasis. This study aimed to evaluate the effects of adalimumab and cyclosporine A on the expression of histaminergic system-related genes and miRNAs regulating these genes in bacterial lipopolysaccharide A (LPS)-stimulated human keratinocyte (HaCaT) cells. HaCaT cells were treated with 1 µg/mL LPS for 8 h, followed by treatment with 8 µg/mL adalimumab or 100 ng/mL cyclosporine A for 2, 8, or 24 h. Untreated cells served as controls. The cells were subjected to ribonucleic acid (RNA) extraction and microarray, quantitative real-time polymerase chain reaction, and enzyme-linked immunosorbent assay analyses. Statistical analysis was performed using the Statistica 13.0 PL (StatSoft, Cracow, Poland) and the Transcriptome Analysis Console programs (Affymetrix, Santa Clara, CA, USA) (

Indexed as

MicroRNAsPsoriasisAdalimumabAdultCyclosporinegamma-Aminobutyric AcidHaCaT CellsHistamine N-MethyltransferaseHumansLipopolysaccharidesMatrix Metalloproteinase 2Phosphatidylinositol 3-KinasesPolycomb Repressive Complex 1Transforming Growth Factor betaTumor Necrosis Factor-alphaAdalimumabCBX7 protein, humanCyclosporinegamma-Aminobutyric AcidHistamine N-MethyltransferaseLipopolysaccharidesMatrix Metalloproteinase 2MicroRNAsMIRN1275 microRNA, humanPhosphatidylinositol 3-KinasesPolycomb Repressive Complex 1Transforming Growth Factor betaTumor Necrosis Factor-alphaAdalimumabcyclosporine AHaCaThistaminergic systemlipopolysaccharide AmiRNAmRNAproteinpsoriasis

Identifiers

PMID35899934
PMCPMC9678002
OpenAlexW4288084699

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.