ArticlePlant, cell & environment2022
Exact mass GC-MS analysis: Protocol, database, advantages and application to plant metabolic profiling.
Article in Plant, cell & environment, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Non-Targeted Metabolomic Analyses Provide Insights into Exogenous Trehalose-Mediated Heat Stress Tolerance in Tea Plants (Plants (Basel, Switzerland) · 2026Article
- Analyzing Plant Low-Molecular-Weight Polar Metabolites: A GC-MS Approach.Plants (Basel, Switzerland) · 2026Review
- Response mechanism of extracellular polymers in the remediation of chromium pollution by carbonate mineralizing bacteria.RSC advances · 2025Article
- Effect of clove flower extract (Narra J · 2025Article
- Metabolomic Characterization and Bioinformatic Studies of Bioactive Compounds in Two Varieties ofInternational journal of molecular sciences · 2025Article
- Comprehensive Analysis of Metabolic Changes in Mice Exposed to Corilagin Based on GC-MS Analysis.Drug design, development and therapy · 2025Article
- Unlocking the pharmacological potential of Brennnesselwurzel (Urtica dioica L.): an in-depth study on multifaceted biological activities.BMC complementary medicine and therapies · 2024Article
- Metabolomics-Assisted Breeding in Oil Palm: Potential and Current Perspectives.International journal of molecular sciences · 2024Review
- Variation in leaf dark respiration among C3 and C4 grasses is associated with use of different substrates.Plant physiology · 2024Article
- Combining Gas Exchange and Rapid Quenching of Leaf Tissue for Mass Spectrometry and NMR Analysis Using an External Chamber.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Evaluation of GC/MS-BasedMetabolites · 2023Article
- Exact mass GC-MS analysis: Protocol, database, advantages and application to plant metabolic profiling.Plant, cell & environment · 2022Article
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3 authors.
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Abstract
Plant metabolomics has been used widely in plant physiology, in particular to analyse metabolic responses to environmental parameters. Derivatization (via trimethylsilylation and methoximation) followed by GC-MS metabolic profiling is a major technique to quantify low molecular weight, common metabolites of primary carbon, sulphur and nitrogen metabolism. There are now excellent opportunities for new generation analyses, using high resolution, exact mass GC-MS spectrometers that are progressively becoming relatively cheap. However, exact mass GC-MS analyses for routine metabolic profiling are not common, since there is no dedicated available database. Also, exact mass GC-MS is usually dedicated to structural resolution of targeted secondary metabolites. Here, we present a curated database for exact mass metabolic profiling (made of 336 analytes, 1064 characteristic exact mass fragments) focused on molecules of primary metabolism. We show advantages of exact mass analyses, in particular to resolve isotopic patterns, localise S-containing metabolites, and avoid identification errors when analytes have common nominal mass peaks in their spectrum. We provide a practical example using leaves of different Arabidopsis ecotypes and show how exact mass GC-MS analysis can be applied to plant samples and identify metabolic profiles.
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