ArticleDevelopment (Cambridge, England)2022
Rapid and robust directed differentiation of mouse epiblast stem cells into definitive endoderm and forebrain organoids.
Article in Development (Cambridge, England), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
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Who cites it
17 citing papers in PubMed, 1 synthesis or guideline pooled it, 27 citations in OpenAlex.
- Mapping the Cerebral Organoid Landscape: A Systematic Review of Preclinical 3D Models in Neuroscience.Advanced healthcare materials · 2026Pooled it
- Brain Organoids: Emerging Platforms for Modern Neuroscience.Brain sciences · 2026Review
- Establishing mouse forebrain organoids as models of intrinsic cortical network assembly.Stem cell reports · 2026Article
- Independent control of neurogenesis and dorsoventral patterning by NKX2-2.Genes & development · 2026Article
- NIPBL-mediated 3D genome folding translates enhancer priming into gene activation and safeguards lineage fidelity during embryonic transitions.bioRxiv : the preprint server for biology · 2025Article
- A mouse organoid platform for modeling cerebral cortex development and cis-regulatory evolution in vitro.Developmental cell · 2025Article
- Enhancer remodeling by OTX2 directs specification and patterning of mammalian definitive endoderm.Developmental cell · 2025Article
- Genome-wide CRISPR screen identifies Menin and SUZ12 as regulators of human developmental timing.Nature cell biology · 2025Article
- Transcription factors SP5 and SP8 drive primary cilia formation in mammalian embryos.Science (New York, N.Y.) · 2025Article
- MODELING TISSUE-RESIDENT MACROPHAGE DEVELOPMENT FROM MOUSE PLURIPOTENT STEM CELLS.bioRxiv : the preprint server for biology · 2025Article
- A single-cell atlas of spatial and temporal gene expression in the mouse cranial neural plate.eLife · 2025Article
- Established and emerging new approach methodologies in neuroscience.Frontiers in neuroscience · 2025Review
- SIRV: spatial inference of RNA velocity at the single-cell resolution.NAR genomics and bioinformatics · 2024Article
- A PLURIPOTENT STEM CELL PLATFORM FOR IN VITRO SYSTEMS GENETICS STUDIES OF MOUSE DEVELOPMENT.bioRxiv : the preprint server for biology · 2024Article
- Article
- Chromatin regulation of transcriptional enhancers and cell fate by the Sotos syndrome gene NSD1.Molecular cell · 2023Article
- Systematic mapping and modeling of 3D enhancer-promoter interactions in early mouse embryonic lineages reveal regulatory principles that determine the levels and cell-type specificity of gene expression.bioRxiv : the preprint server for biology · 2023Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Directed differentiation of pluripotent stem cells (PSCs) is a powerful model system for deconstructing embryonic development. Although mice are the most advanced mammalian model system for genetic studies of embryonic development, state-of-the-art protocols for directed differentiation of mouse PSCs into defined lineages require additional steps and generates target cell types with lower purity than analogous protocols for human PSCs, limiting their application as models for mechanistic studies of development. Here, we examine the potential of mouse epiblast stem cells cultured in media containing Wnt pathway inhibitors as a starting point for directed differentiation. As a proof of concept, we focused our efforts on two specific cell/tissue types that have proven difficult to generate efficiently and reproducibly from mouse embryonic stem cells: definitive endoderm and neural organoids. We present new protocols for rapid generation of nearly pure definitive endoderm and forebrain-patterned neural organoids that model the development of prethalamic and hippocampal neurons. These differentiation models present new possibilities for combining mouse genetic tools with in vitro differentiation to characterize molecular and cellular mechanisms of embryonic development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.