ArticleMolecular ecology resources2023
Environmental DNA methylation of Lymnaea stagnalis varies with age and is hypermethylated compared to tissue DNA.
Article in Molecular ecology resources, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Prospects of multipurpose biomonitoring for fisheries assessment based on environmental nucleic acids.Journal of fish biology · 2026Review
- The potential of methylation signal detection in eDNA toward functional ecological monitoring.Communications biology · 2026Review
- A Field-Deployable eDNA Metabarcoding Workflow Including De Novo Reference Assembly for Characterising Understudied Biodiversity Hotspots.Molecular ecology resources · 2026Article
- Unlocking Demography: Developing an eDNA-Based Toolkit to Measure Sex Ratios From Populations.Molecular ecology resources · 2026Article
- Environmental DNA Epigenetics Accurately Predicts the Age of Cultured Fish Larvae.Ecology and evolution · 2025Article
- Article
- Improved environmental DNA detection sensitivity of Opisthorchis viverrini using a multi-marker assay.Parasitology research · 2024Article
- An epigenetic toolbox for conservation biologists.Evolutionary applications · 2024Article
- DNA Methylation Machinery in Gastropod Mollusks.Life (Basel, Switzerland) · 2024Review
- Mixed Patterns of Intergenerational DNA Methylation Inheritance in Acropora.Molecular biology and evolution · 2024Article
- Environmental DNA methylation of Lymnaea stagnalis varies with age and is hypermethylated compared to tissue DNA.Molecular ecology resources · 2023Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Environmental DNA (eDNA) approaches contributing to species identifications are quickly becoming the new norm in biomonitoring and ecosystem assessments. Yet, information such as age and health state of the population, which is vital to species biomonitoring, has not been accessible from eDNA. DNA methylation has the potential to provide such information on the state of a population. Here, we measured the methylation of eDNA along with tissue DNA (tDNA) of Lymnaea stagnalis at four life stages. We demonstrate that eDNA methylation varies with age and allows distinguishing among age classes. Moreover, eDNA was globally hypermethylated in comparison to tDNA. This difference was age-specific and connected to a limited number of eDNA sites. This differential methylation pattern suggests that eDNA release with age is partially regulated through DNA methylation. Our findings help to understand mechanisms involved in eDNA release and shows the potential of eDNA methylation analysis to assess age classes. Such age class assessments will encourage future eDNA studies to assess fundamental processes of population dynamics and functioning in ecology, biodiversity conservation and impact assessments.
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