ArticleFrontiers in veterinary science2022
CRISPR-Cas13a Based Visual Detection Assays for Feline Calicivirus Circulating in Southwest China.
Article in Frontiers in veterinary science, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 15 citations in OpenAlex.
- Development and Application of a Blocking ELISA for the Detection of Feline Calicivirus Antibodies Based on Monoclonal Antibodies Against VP1 Protein.Transboundary and emerging diseases · 2026Article
- Recent advances in rapid multiplex detection of nucleic acid markers using RPA and CRISPR-Cas.Frontiers in microbiology · 2026Review
- Integrated CRISPR-Cas12a and RAA one-pot visual strategy for the rapid identification ofFrontiers in cellular and infection microbiology · 2025Article
- Clinical diagnostic value of CRISPR-Cas13a-based molecular technology for tuberculosis.Irish journal of medical science · 2024Article
- Article
- Ultrasensitive and visual detection of Feline herpesvirus type-1 and Feline calicivirus using one-tube dRPA-Cas12a/Cas13a assay.BMC veterinary research · 2024Article
- Update on feline calicivirus: viral evolution, pathogenesis, epidemiology, prevention and control.Frontiers in microbiology · 2024Review
- Development and application of a dual ERA method for the detection of Feline Calicivirus and Feline Herpesvirus Type I.Virology journal · 2023Article
- Recent Advances in CRISPR-Cas Technologies for Synthetic Biology.Journal of microbiology (Seoul, Korea) · 2023Review
- Application of the CRISPR/Cas System in Pathogen Detection: A Review.Molecules (Basel, Switzerland) · 2022Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Feline calicivirus (FCV) is a well-known causative pathogen for upper respiratory infection in cats. Its high genetic variability challenges existing molecular diagnostic methods in clinical settings. Thus, we developed two sensitive and visual assays for FCV nucleic acid detection based on RPA reaction and CRISPR-Cas13a trans-cleavage activity. Recombinant plasmid DNA, crRNAs, and RPA primers were designed and prepared, respectively, targeting to FCV ORF1 gene. Besides, purified LwCas13a protein was produced by
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Registered trials
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