Evidence map›Paper›PMID 35898416›Full record

ArticleInternational journal of food science2022

Molecular Characterization and Diversity of Bacteria Isolated from Fish and Fish Products Retailed in Kenyan Markets.

Domitila Ndinda Kyule, John M Maingi, Ezekiel Mugendi Njeru, Anthony Kebira Nyamache

Open access · goldAbstract read
In one paragraph

Article in International journal of food science, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.5field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 17 citations in OpenAlex.

  1. Article
  2. Review
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  4. Article
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  6. Prevalence and Antimicrobial Resistance Diversity ofAntibiotics (Basel, Switzerland) · 2024
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Domitila Ndinda KyuleDepartment of Biochemistry, Microbiology and Biotechnology, Kenyatta University, 43844-00100 Nairobi, Kenya.ORCID https://orcid.org/0000-0003-1264-3743
John M MaingiDepartment of Biochemistry, Microbiology and Biotechnology, Kenyatta University, 43844-00100 Nairobi, Kenya.ORCID https://orcid.org/0000-0002-9314-5115
Ezekiel Mugendi NjeruDepartment of Biochemistry, Microbiology and Biotechnology, Kenyatta University, 43844-00100 Nairobi, Kenya.ORCID https://orcid.org/0000-0002-9104-808X
Anthony Kebira NyamacheDepartment of Biochemistry, Microbiology and Biotechnology, Kenyatta University, 43844-00100 Nairobi, Kenya.ORCID https://orcid.org/0000-0002-6168-881X
Kenyatta University · KE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Fish products are highly vulnerable to microbial contamination due to their soft tissues, making them perishable and harmful to consumers. The clinical and subclinical infections reported by fish consumers are mainly associated with pathogenic microorganisms in fish products. Therefore, this study aimed at establishing the molecular profiles and diversity of the bacterial isolates from fish and fish products obtained from Kirinyaga County markets in Kenya. A total of 660 samples were randomly sampled in six Kirinyaga County markets and transported to Kenyatta University for bacterial isolation. The fish skin surface was cut using a sterile knife and blended in buffered peptone water. The blended product was serially diluted and plated on nutrient agar. After 24 hours, the bacteria cultures were subcultured to obtain pure bacterial isolates. The pure isolates were grouped and characterized based on their morphology and biochemical characteristics. One representative of each group was selected for bacterial DNA extraction. The 16S rRNA gene was amplified using the 27F and 1492R primers, and the obtained PCR product was subjected to Sanger-based sequencing using the same primers. Morphological characterization yielded 54 morpho groups. Phylogenetic analysis revealed diverse bacterial strains, including

Identifiers

PMID35898416
PMCPMC9313967
OpenAlexW4285729158

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.