Evidence map›Paper›PMID 35896564›Full record

ArticleScientific data2022

Standard metadata for 3D microscopy.

Alexander J Ropelewski, Megan A Rizzo, Jason R Swedlow, Jan Huisken, Pavel Osten, Neda Khanjani, Kurt Weiss, Vesselina Bakalov, Michelle Engle, Lauren Gridley and 8 more

Abstract read
In one paragraph

Article in Scientific data, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Review
  3. Article
  4. Artificial Neural Networks for Bioimage Analysis.Methods in molecular biology (Clifton, N.J.) · 2026
    Article
  5. Article
  6. Article
  7. Review
  8. Article
  9. Article
  10. Article
  11. Article
  12. T-CLEARE: a pilot community-driven tissue clearing protocol repository.Frontiers in bioengineering and biotechnology · 2024
    Article
  13. Review
  14. Article
  15. Review
  16. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors.

Alexander J RopelewskiBiomedical Applications Group, Pittsburgh Supercomputing Center, 300 S Craig Street, Pittsburgh, PA, 15213, USA.
Megan A RizzoDepartment of Physiology, University of Maryland School of Medicine, 660 West Redwood Street, Baltimore, MD, 21201, USA.ORCID 0000-0001-6528-7768
Jason R SwedlowCentre for Gene Regulation & Expression, Division of Computational Biology, University of Dundee, Nethergate, Dundee, Scotland, DD1 4HN, United Kingdom.ORCID 0000-0002-2198-1958
Jan HuiskenMorgridge Institute for Research, 330 N Orchard Street, Madison, WI, 53715, USA.ORCID 0000-0001-7250-3756
Pavel OstenCold Spring Harbor Laboratory, One Bungtown Road, Cold Spring Harbor, NY, 11724, USA.ORCID 0000-0002-6385-7541
Neda KhanjaniMark and Mary Stevens Neuroimaging and Informatics Institute, Laboratory of Neuro Imaging, Keck School of Medicine of University of Southern California, 1975 Zonal Avenue, Los Angeles, CA, 90033, USA.
Kurt WeissMorgridge Institute for Research, 330 N Orchard Street, Madison, WI, 53715, USA.
Vesselina BakalovBioinformatics and Computational Biology Program, RTI International, 3040 East Cornwallis Road, Research Triangle Park, NC, 27709, USA.
Michelle EngleBioinformatics and Computational Biology Program, RTI International, 3040 East Cornwallis Road, Research Triangle Park, NC, 27709, USA.ORCID 0000-0002-4931-8541
Lauren GridleyBioinformatics and Computational Biology Program, RTI International, 3040 East Cornwallis Road, Research Triangle Park, NC, 27709, USA.
Michelle KrzyzanowskiBioinformatics and Computational Biology Program, RTI International, 3040 East Cornwallis Road, Research Triangle Park, NC, 27709, USA.
Tom MaddenBioinformatics and Computational Biology Program, RTI International, 3040 East Cornwallis Road, Research Triangle Park, NC, 27709, USA.
Deborah MaieseBioinformatics and Computational Biology Program, RTI International, 3040 East Cornwallis Road, Research Triangle Park, NC, 27709, USA.
Meisha MandalBioinformatics and Computational Biology Program, RTI International, 3040 East Cornwallis Road, Research Triangle Park, NC, 27709, USA.
Justin WaterfieldBioinformatics and Computational Biology Program, RTI International, 3040 East Cornwallis Road, Research Triangle Park, NC, 27709, USA.ORCID 0000-0001-6716-2897
David WilliamsBioinformatics and Computational Biology Program, RTI International, 3040 East Cornwallis Road, Research Triangle Park, NC, 27709, USA.
Carol M HamiltonBioinformatics and Computational Biology Program, RTI International, 3040 East Cornwallis Road, Research Triangle Park, NC, 27709, USA.
Wayne HugginsBioinformatics and Computational Biology Program, RTI International, 3040 East Cornwallis Road, Research Triangle Park, NC, 27709, USA. whuggins@rti.org.ORCID 0000-0002-1463-3883

Funding

Collaboratory for atlasing cell type anatomy in the female and male mouse brainU01MH114824 · NIMH · COLD SPRING HARBOR LABORATORY · PI MITRA, PARTHA PRATIM · 2017 to 2021
$11.7M
A Confocal Fluorescence Microscopy Brain Data ArchiveR24MH114793 · NIMH · CARNEGIE-MELLON UNIVERSITY · PI ALEXANDER J ROPELEWSKI, Alan Michael Watson · 2017 to 2026
$10.1M
Collaborative Standards for Brain MicroscopyR24MH114683 · NIMH · RESEARCH TRIANGLE INSTITUTE · PI HUGGINS, BRUCE WAYNE · 2018 to 2019
$1.0M
NIMH NIH HHS R24 MH114683NIMH NIH HHS R24 MH114793NIMH NIH HHS U01 MH114824
6 · The paper itself

Abstract

Recent advances in fluorescence microscopy techniques and tissue clearing, labeling, and staining provide unprecedented opportunities to investigate brain structure and function. These experiments' images make it possible to catalog brain cell types and define their location, morphology, and connectivity in a native context, leading to a better understanding of normal development and disease etiology. Consistent annotation of metadata is needed to provide the context necessary to understand, reuse, and integrate these data. This report describes an effort to establish metadata standards for three-dimensional (3D) microscopy datasets for use by the Brain Research through Advancing Innovative Neurotechnologies® (BRAIN) Initiative and the neuroscience research community. These standards were built on existing efforts and developed with input from the brain microscopy community to promote adoption. The resulting 3D Microscopy Metadata Standards (3D-MMS) includes 91 fields organized into seven categories: Contributors, Funders, Publication, Instrument, Dataset, Specimen, and Image. Adoption of these metadata standards will ensure that investigators receive credit for their work, promote data reuse, facilitate downstream analysis of shared data, and encourage collaboration.

Indexed as

MetadataMicroscopyBrainDatasets as TopicHumans

Identifiers

PMID35896564
PMCPMC9329339

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.