Evidence map›Paper›PMID 35896096›Full record

ReviewJournal of microscopy2022

Fluorescence fluctuation-based super-resolution microscopy: Basic concepts for an easy start.

Alma Alva, Eduardo Brito-Alarcón, Alejandro Linares, Esley Torres-García, Haydee O Hernández, Raúl Pinto-Cámara, Damián Martínez, Paul Hernández-Herrera, Rocco D'Antuono, Christopher Wood and 1 more

Abstract readReview
In one paragraph

Review in Journal of microscopy, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Review
  6. Article
  7. Review
  8. Review
  9. Article
  10. Wide-field intensity fluctuation imaging.Biomedical optics express · 2024
    Article
  11. Article
  12. Wide-field Intensity Fluctuation Imaging.bioRxiv : the preprint server for biology · 2023
    Article
  13. Article
  14. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Alma AlvaLaboratorio Nacional de Microscopía Avanzada, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico.ORCID 0000-0001-8676-8253
Eduardo Brito-AlarcónLaboratorio Nacional de Microscopía Avanzada, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico.ORCID 0000-0001-5209-5388
Alejandro LinaresLaboratorio Nacional de Microscopía Avanzada, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico.
Esley Torres-GarcíaLaboratorio Nacional de Microscopía Avanzada, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico.
Haydee O HernándezPosgrado en Ciencia e Ingeniería de la Computación, Universidad Nacional Autónoma de México, Mexico City, Mexico.
Raúl Pinto-CámaraLaboratorio Nacional de Microscopía Avanzada, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico.
Damián MartínezLaboratorio Nacional de Microscopía Avanzada, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico.
Paul Hernández-HerreraLaboratorio Nacional de Microscopía Avanzada, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico.
Rocco D'AntuonoCrick Advanced Light Microscopy Science and Technology Platform, The Francis Crick Institute, London, UK.ORCID 0000-0003-0180-6500
Christopher WoodLaboratorio Nacional de Microscopía Avanzada, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico.
Adán GuerreroLaboratorio Nacional de Microscopía Avanzada, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico.

Funding

Medical Research Council FC001999Wellcome Trust FC001999
6 · The paper itself

Abstract

Due to the wave nature of light, optical microscopy has a lower-bound lateral resolution limit of approximately half of the wavelength of visible light, that is, within the range of 200 to 350 nm. Fluorescence fluctuation-based super-resolution microscopy (FF-SRM) is a term used to encompass a collection of image analysis techniques that rely on the statistical processing of temporal variations of the fluorescence signal. FF-SRM aims to reduce the uncertainty of the location of fluorophores within an image, often improving spatial resolution by several tens of nanometers. FF-SRM is suitable for live-cell imaging due to its compatibility with most fluorescent probes and relatively simple instrumental and experimental requirements, which are mostly camera-based epifluorescence instruments. Each FF-SRM approach has strengths and weaknesses, which depend directly on the underlying statistical principles through which enhanced spatial resolution is achieved. In this review, the basic concepts and principles behind a range of FF-SRM methods published to date are described. Their operational parameters are explained and guidance for their selection is provided.

Indexed as

Fluorescent DyesImage Processing, Computer-AssistedMicroscopy, FluorescenceFluorescent Dyesbioimagingcomputational biologyfluorescence fluctuationfluorescence microscopynanoscopysuper-resolution microscopy

Identifiers

PMID35896096
PMCPMC10087389

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.