Evidence map›Paper›PMID 35896060›Full record

ArticleEnvironmental science & technology2022

Horizontal Gene Transfer of an IncP1 Plasmid to Soil Bacterial Community Introduced by

Gonçalo Macedo, Asmus K Olesen, Lorrie Maccario, Lucia Hernandez Leal, Peter V D Maas, Dick Heederik, Dik Mevius, Søren J Sørensen, Heike Schmitt

Open access · hybridAbstract read
In one paragraph

Article in Environmental science & technology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
5.4field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 37 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
  4. Review
  5. Article
  6. Article
  7. Article
  8. Article
  9. Assessing horizontal gene transfer in the rhizosphere ofApplied and environmental microbiology · 2024
    Article
  10. Article
  11. Article
  12. Article
  13. Review
  14. Development of a high-throughput platform to measure plasmid transfer frequency.Frontiers in cellular and infection microbiology · 2023
    Article
  15. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 2 countries.

Gonçalo MacedoDepartment of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, 3584 CL Utrecht, The Netherlands.ORCID 0000-0003-3595-4018
Asmus K OlesenDepartment of Biology, University of Copenhagen, Copenhagen 2100, Denmark.
Lorrie MaccarioDepartment of Biology, University of Copenhagen, Copenhagen 2100, Denmark.
Lucia Hernandez LealWetsus, European Centre of Excellence for Sustainable Water Technology, Oostergoweg 9, 8911 MA Leeuwarden, The Netherlands.
Peter V D MaasVan Hall Larenstein, University of Applied Sciences, Agora 1, 8901 BV Leeuwarden, The Netherlands.
Dick HeederikInstitute for Risk Assessment Sciences, Utrecht University, Yalelaan 2, 3584 CM Utrecht, The Netherlands.
Dik MeviusDepartment of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, 3584 CL Utrecht, The Netherlands.
Søren J SørensenDepartment of Biology, University of Copenhagen, Copenhagen 2100, Denmark.
Heike SchmittWetsus, European Centre of Excellence for Sustainable Water Technology, Oostergoweg 9, 8911 MA Leeuwarden, The Netherlands.ORCID 0000-0002-0139-1934
Utrecht University · NLUniversity of Copenhagen · DKVan Hall Larenstein University of Applied Sciences · NL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The quantification and identification of new plasmid-acquiring bacteria in representative mating conditions is critical to characterize the risk of horizontal gene transfer in the environment. This study aimed to quantify conjugation events resulting from manure application to soils and identify the transconjugants resulting from these events. Conjugation was quantified at multiple time points by plating and flow cytometry, and the transconjugants were recovered by fluorescence-activated cell sorting and identified by 16S rRNA sequencing. Overall, transconjugants were only observed within the first 4 days after manure application and at values close to the detection limits of this experimental system (1.00-2.49 log CFU/g of manured soil, ranging between 10

Indexed as

ManureSoilAnti-Bacterial AgentsBacteriaEscherichia coliGene Transfer, HorizontalPlasmidsRNA, Ribosomal, 16SSoil MicrobiologyAnti-Bacterial AgentsManureRNA, Ribosomal, 16SSoilantibiotic resistance genecattle manurelateral gene transfermatingsoil microbiome

Identifiers

PMID35896060
PMCPMC9387108
OpenAlexW4288067781

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.