ArticleVeterinary medicine and science2022
Three-dimensional in vitro maturation of rabbit oocytes enriched with sheep decellularized greater omentum.
Article in Veterinary medicine and science, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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4 citing papers in PubMed, 10 citations in OpenAlex.
- Conditioned media and extracellular vesicles derived from human Wharton's jelly mesenchymal stem cells improve the in vitro maturation of immature oocytes in normal and PCOS mouse model.Biomedical engineering online · 2025Article
- Endothelial cell supplementation promotes xenograft revascularization during short-term ovarian tissue transplantation.Bioactive materials · 2025Article
- Extracellular matrix-derived scaffolds in constructing artificial ovaries for ovarian failure: a systematic methodological review.Human reproduction open · 2023Review
- Three-dimensional in vitro maturation of rabbit oocytes enriched with sheep decellularized greater omentum.Veterinary medicine and science · 2022Article
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Authors and funding
3 authors at 1 institution in 1 country.
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Abstract
backgroundTo prevent ovarian hyperstimulation syndrome, in vitro maturation (IVM) allows the oocytes for infertility treatment without hormone therapy. Although many oocytes matured during IVM, some deficiencies in the culture conditions lead to inhibition of the growth and development of the cumulus cells and the oocyte nuclear and cytoplasmic maturation.
objectivesThe challenge of improving the oocyte culture conditions prompted us to use greater omentum (GOM), full of growth factors and proteins, as a rich supplement to the base culture medium.
methodsCumulus-oocyte complexes were recovered from rabbits and divided into 3D and 2D conditions cultured for 12 and 24 h. In 3D cultures, the oocytes embedded in alginate containing FBS decellularized GOM. Corresponding supplements were also added in 2D conditions-maturation of the oocytes evaluated by Aceto-Orcein, TEM, and RT-PCR for MAP2K1 and Cdk2.
resultsDNA quantification, Hoechst, and H&E staining confirmed cell depletion from GOM, and SEM showed the preservation of ultra-architecture after decellularization. Histochemical staining methods showed appropriate extracellular matrix preservation. ELISA assessment showed retention of VEGF content. MTT assessment indicated decellularized GOM was non-toxic. Both Aceto-Orcein assessment and ultra-structure study of the oocytes showed that supplementation of 2D or 3D cultures with decellularized omentum promoted oocyte maturation. Expression of MAP2K1 and Cdk2 also increased in the presence of GOM.
conclusionsGOM supplementation has a beneficial impact on oocyte maturation, probably due to the presence of growth factors and proteins.
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