ArticleMethods in molecular biology (Clifton, N.J.)2022
In Situ Imaging of mRNA Splicing Variants by SpliceRCA.
Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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2 authors at 2 institutions in 1 country.
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Abstract
We present a method for multiplexed in situ imaging of individual RNA splicing variants. This method enables quantifying RNA splicing variants with single-molecule resolution, discriminating splicing isoforms with single-base precision as well as analyzing the subcellular localization of transcripts. With this technology, it is possible to study cell heterogeneity of gene expression, potentially helping to decipher rich diversity in posttranscriptional function and assist clinical monitoring.
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