Evidence map›Paper›PMID 35895265›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

BaseScope™ Approach to Visualize Alternative Splice Variants in Tissue.

Alexandra Bunda, Arturo Andrade

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.5field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. bioRxiv : the preprint server for biology · 2026
    Article
  3. Inactivation of CaV1 and CaV2 channels.The Journal of general physiology · 2025
    Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 2 institutions in 1 country.

Alexandra BundaDepartment of Biological Sciences, College of Life Sciences and Agriculture, University of New Hampshire, Durham, NH, USA.
Arturo AndradeDepartment of Biological Sciences, College of Life Sciences and Agriculture, University of New Hampshire, Durham, NH, USA. Arturo_Andrade@brown.edu.
Allen Institute for Brain Science · USUniversity of New Hampshire · US

Funding

Role of Gi/o-Protein Inhibition of CaV2.2 Channels in Anxiety BehaviorR00MH099405 · NIMH · UNIVERSITY OF NEW HAMPSHIRE · PI ANDRADE, ARTURO · 2015 to 2017
$738k
NIMH NIH HHS R00 MH099405
6 · The paper itself

Abstract

Defining the cell-specific alternative splicing landscape in complex tissues is an important goal to gain functional insights. Deep-sequencing techniques coupled to genetic strategies for cell identification has provided important cues on cell-specific exon usage in complex tissues like the nervous system. BaseScope™ has emerged as a powerful and highly sensitive alternative to in situ hybridization to determine exon composition in tissue with spatial and morphological context. In this protocol, we will review how BaseScope was utilized to detect the e37a-Cacna1b splice variant of the presynaptic calcium channel Ca

Indexed as

Alternative SplicingCalcium Channels, N-TypeExonsIn Situ HybridizationRNA, MessengerCalcium Channels, N-TypeRNA, MessengerAlternative splicingBaseScope™Cacna1bCaV2.2In situ hybridizationN-type

Identifiers

PMID35895265
OpenAlexW4288064169

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.