Evidence map›Paper›PMID 35893246›Full record

ArticleMetabolites2022

Preanalytical Pitfalls in Untargeted Plasma Nuclear Magnetic Resonance Metabolomics of Endocrine Hypertension.

Nikolaos G Bliziotis, Leo A J Kluijtmans, Gerjen H Tinnevelt, Parminder Reel, Smarti Reel, Katharina Langton, Mercedes Robledo, Christina Pamporaki, Alessio Pecori, Josie Van Kralingen and 28 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Metabolites, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.6field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

38 authors at 15 institutions in 12 countries.

Nikolaos G BliziotisDepartment of Laboratory Medicine, Translational Metabolic Laboratory, Radboud University Medical Center, 6525 GA Nijmegen, The Netherlands.
Leo A J KluijtmansDepartment of Laboratory Medicine, Translational Metabolic Laboratory, Radboud University Medical Center, 6525 GA Nijmegen, The Netherlands.ORCID 0000-0002-1999-3378
Gerjen H TinneveltDepartment of Analytical Chemistry, Institute for Molecules and Materials, Radboud University, 6500 HB Nijmegen, The Netherlands.
Parminder ReelDivision of Population Health and Genomics, School of Medicine, University of Dundee, Dundee DD2 4BF, UK.ORCID 0000-0001-7796-1457
Smarti ReelDivision of Population Health and Genomics, School of Medicine, University of Dundee, Dundee DD2 4BF, UK.
Katharina LangtonDepartment of Medicine III, University Hospital Carl Gustav Carus, Technische Universität Dresden, 01307 Dresden, Germany.
Mercedes RobledoHereditary Endocrine Cancer Group, Spanish National Cancer Research Centre (CNIO), Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBERER), 28029 Madrid, Spain.ORCID 0000-0001-6256-5902
Christina PamporakiDepartment of Medicine III, University Hospital Carl Gustav Carus, Technische Universität Dresden, 01307 Dresden, Germany.
Alessio PecoriDivision of Internal Medicine and Hypertension Unit, Department of Medical Sciences, University of Torino, 10124 Torino, Italy.
Josie Van KralingenBritish Heart Foundation Glasgow Cardiovascular Research Centre (BHF GCRC), Institute of Cardiovascular & Medical Sciences (ICAMS), University of Glasgow, Glasgow G12 8TA, UK.
Martina TettiDivision of Internal Medicine and Hypertension Unit, Department of Medical Sciences, University of Torino, 10124 Torino, Italy.
Udo F H EngelkeDepartment of Laboratory Medicine, Translational Metabolic Laboratory, Radboud University Medical Center, 6525 GA Nijmegen, The Netherlands.
Zoran ErlicDepartment of Endocrinology, Diabetology and Clinical Nutrition, University Hospital Zurich (USZ) and University of Zurich (UZH), 8091 Zurich, Switzerland.ORCID 0000-0001-6953-9133
Jasper EngelBiometris, Wageningen University & Research, 6708 PB Wageningen, The Netherlands.
Timo DeutschbeinDepartment of Internal Medicine I, Division of Endocrinology and Diabetes, University Hospital, University of Würzburg, 97080 Wurzburg, Germany.
Svenja NöltingDepartment of Medicine IV, University Hospital, LMU Munich, 80336 Munich, Germany.
Aleksander PrejbiszDepartment of Hypertension, Institute of Cardiology, 04-628 Warsaw, Poland.
Susan RichterInstitute of Clinical Chemistry and Laboratory Medicine, University Hospital Carl Gustav Carus at the Technische Universität Dresden, 01307 Dresden, Germany.ORCID 0000-0002-3549-2477
Jerzy AdamskiResearch Unit Molecular Endocrinology and Metabolism, Genome Analysis Center, Helmholtz Center München, German Research Center for Environmental Health, 85764 Neuherberg, Germany.ORCID 0000-0001-9259-0199
Andrzej JanuszewiczDepartment of Hypertension, Institute of Cardiology, 04-628 Warsaw, Poland.
Filippo CeccatoEndocrinology Unit, Department of Medicine DIMED, University-Hospital of Padova, 35128 Padova, Italy.ORCID 0000-0003-1456-8716
Carla ScaroniEndocrinology Unit, Department of Medicine DIMED, University-Hospital of Padova, 35128 Padova, Italy.
Michael C DennedyThe Discipline of Pharmacology and Therapeutics, School of Medicine, National University of Ireland, H91 CF50 Galway, Ireland.
Tracy A WilliamsDivision of Internal Medicine and Hypertension Unit, Department of Medical Sciences, University of Torino, 10124 Torino, Italy.
Livia LenziniDepartment of Medicine-DIMED, Emergency and Hypertension Unit, University of Padova, University Hospital, 35126 Padova, Italy.
Anne-Paule Gimenez-RoqueploINSERM, PARCC, Université de Paris, 75015 Paris, France.ORCID 0000-0002-4816-670X
Eleanor DaviesBritish Heart Foundation Glasgow Cardiovascular Research Centre (BHF GCRC), Institute of Cardiovascular & Medical Sciences (ICAMS), University of Glasgow, Glasgow G12 8TA, UK.
Martin FassnachtDepartment of Internal Medicine I, Division of Endocrinology and Diabetes, University Hospital, University of Würzburg, 97080 Wurzburg, Germany.ORCID 0000-0001-6170-6398
Hanna RemdeDepartment of Internal Medicine I, Division of Endocrinology and Diabetes, University Hospital, University of Würzburg, 97080 Wurzburg, Germany.
Graeme EisenhoferDepartment of Medicine III, University Hospital Carl Gustav Carus, Technische Universität Dresden, 01307 Dresden, Germany.
Felix BeuschleinDepartment of Endocrinology, Diabetology and Clinical Nutrition, University Hospital Zurich (USZ) and University of Zurich (UZH), 8091 Zurich, Switzerland.
Matthias KroissDepartment of Internal Medicine I, Division of Endocrinology and Diabetes, University Hospital, University of Würzburg, 97080 Wurzburg, Germany.ORCID 0000-0002-7628-3161
Emily JeffersonDivision of Population Health and Genomics, School of Medicine, University of Dundee, Dundee DD2 4BF, UK.ORCID 0000-0003-2992-7582
Maria-Christina ZennaroINSERM, PARCC, Université de Paris, 75015 Paris, France.
Ron A WeversDepartment of Laboratory Medicine, Translational Metabolic Laboratory, Radboud University Medical Center, 6525 GA Nijmegen, The Netherlands.ORCID 0000-0003-2278-9746
Jeroen J JansenDepartment of Analytical Chemistry, Institute for Molecules and Materials, Radboud University, 6500 HB Nijmegen, The Netherlands.
Jaap DeinumDepartment of Internal Medicine, Radboud University Medical Center, 6525 GA Nijmegen, The Netherlands.ORCID 0000-0003-2465-1986
Henri J L M TimmersDepartment of Internal Medicine, Radboud University Medical Center, 6525 GA Nijmegen, The Netherlands.
Radboud University Nijmegen · NLUniversity Hospital Carl Gustav Carus · DEUniversity of Würzburg · DEUniversity of Dundee · GBUniversity of Padua · ITUniversity of Turin · ITBritish Heart Foundation · GBInserm · FRInstitute of Cardiology · PLUniversity of Zurich · CHLMU Klinikum · DENational University of Ireland · IESpanish National Cancer Research Centre · ESUniversity of Ljubljana · SIWageningen University & Research · NL

Funding

European Union 633983
6 · The paper itself

Abstract

Despite considerable morbidity and mortality, numerous cases of endocrine hypertension (EHT) forms, including primary aldosteronism (PA), pheochromocytoma and functional paraganglioma (PPGL), and Cushing's syndrome (CS), remain undetected. We aimed to establish signatures for the different forms of EHT, investigate potentially confounding effects and establish unbiased disease biomarkers. Plasma samples were obtained from 13 biobanks across seven countries and analyzed using untargeted NMR metabolomics. We compared unstratified samples of 106 PHT patients to 231 EHT patients, including 104 PA, 94 PPGL and 33 CS patients. Spectra were subjected to a multivariate statistical comparison of PHT to EHT forms and the associated signatures were obtained. Three approaches were applied to investigate and correct confounding effects. Though we found signatures that could separate PHT from EHT forms, there were also key similarities with the signatures of sample center of origin and sample age. The study design restricted the applicability of the corrections employed. With the samples that were available, no biomarkers for PHT vs. EHT could be identified. The complexity of the confounding effects, evidenced by their robustness to correction approaches, highlighted the need for a consensus on how to deal with variabilities probably attributed to preanalytical factors in retrospective, multicenter metabolomics studies.

Indexed as

confoundersmetabolomicsmulticenterplasma NMRpreanalytical conditions

Identifiers

PMID35893246
PMCPMC9394285
OpenAlexW4287148121

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.