Evidence map›Paper›PMID 35892626›Full record

ArticleAntioxidants (Basel, Switzerland)2022

Experimental Conditions That Influence the Utility of 2'7'-Dichlorodihydrofluorescein Diacetate (DCFH

Lianne R de Haan, Megan J Reiniers, Laurens F Reeskamp, Ali Belkouz, Lei Ao, Shuqun Cheng, Baoyue Ding, Rowan F van Golen, Michal Heger

Abstract read
In one paragraph

Article in Antioxidants (Basel, Switzerland), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Article
  6. Article
  7. AlkylAntioxidants (Basel, Switzerland) · 2025
    Article
  8. Article
  9. The Cyclic Peptide Cyclo-zp80r ControlsFoods (Basel, Switzerland) · 2025
    Article
  10. Review
  11. Article
  12. Enhanced ROS Production and Mitochondrial Metabolic Shifts in CD4International journal of molecular sciences · 2024
    Article
  13. Article
  14. Review
  15. WasteAntioxidants (Basel, Switzerland) · 2023
    Article
  16. On the Photo-Eradication of Methicillin-ResistantInternational journal of molecular sciences · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Lianne R de HaanJiaxing Key Laboratory for Photonanomedicine and Experimental Therapeutics, Department of Pharmaceutics, College of Medicine, Jiaxing University, Jiaxing 314001, China.
Megan J ReiniersJiaxing Key Laboratory for Photonanomedicine and Experimental Therapeutics, Department of Pharmaceutics, College of Medicine, Jiaxing University, Jiaxing 314001, China.
Laurens F ReeskampDepartment of Vascular Medicine, Amsterdam University Medical Centers, Location AMC, 1105 AZ Amsterdam, The Netherlands.ORCID 0000-0002-8043-951X
Ali BelkouzDepartment of Medical Oncology, Amsterdam University Medical Centers, Location AMC, University of Amsterdam, Cancer Center Amsterdam, 1105 AZ Amsterdam, The Netherlands.ORCID 0000-0002-5753-1211
Lei AoJiaxing Key Laboratory for Photonanomedicine and Experimental Therapeutics, Department of Pharmaceutics, College of Medicine, Jiaxing University, Jiaxing 314001, China.
Shuqun ChengDepartment of Hepatic Surgery VI, The Eastern Hepatobiliary Surgery Hospital, The Second Military Medical University, Shanghai 200438, China.
Baoyue DingJiaxing Key Laboratory for Photonanomedicine and Experimental Therapeutics, Department of Pharmaceutics, College of Medicine, Jiaxing University, Jiaxing 314001, China.
Rowan F van GolenDepartment of Gastroenterology and Hepatology, Leiden University Medical Center, 2333 ZA Leiden, The Netherlands.
Michal HegerJiaxing Key Laboratory for Photonanomedicine and Experimental Therapeutics, Department of Pharmaceutics, College of Medicine, Jiaxing University, Jiaxing 314001, China.ORCID 0000-0002-0630-4465

Funding

Basic Public Welfare Research Project of Zhejiang Province LGF18H160034Dutch Cancer Society 10666Grant for the establishment of the Jiaxing Key Laboratory for Photonanomedicine and Experimental Therapeutics 2019National Natural Science Foundation 81872220Tumor Nanotargeting and TCM Technology Innovation Team 2018Zhejiang Foreign Expert Program Grant 2018Zhejiang Provincial Key Natural Science Foundation of China Z20H160031
6 · The paper itself

Abstract

Oxidative stress has been causally linked to various diseases. Electron transport chain (ETC) inhibitors such as rotenone and antimycin A are frequently used in model systems to study oxidative stress. Oxidative stress that is provoked by ETC inhibitors can be visualized using the fluorogenic probe 2',7'-dichlorodihydrofluorescein-diacetate (DCFH

Indexed as

antimycin Aelectron transport chain inhibitorsfluorescence imagingfluorogenic redox probehepatocytesmyxothiazoloxidative stresspiericidin Arotenone

Identifiers

PMID35892626
PMCPMC9329753

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.