Evidence map›Paper›PMID 35891946›Full record

ArticleSynthetic and systems biotechnology2022

A new method for quantitative analysis of M13 bacteriophage by atomic force microscopy.

Yuting Wu, Shuai Liu, Zhiwei Liu, Bing Liu, Bin Du, Zhaoyang Tong, Jianjie Xu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Synthetic and systems biotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 92% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Yuting WuState Key Laboratory of NBC Protection for Civilian, Beijing, 102205, China.
Shuai LiuState Key Laboratory of NBC Protection for Civilian, Beijing, 102205, China.
Zhiwei LiuState Key Laboratory of NBC Protection for Civilian, Beijing, 102205, China.
Bing LiuState Key Laboratory of NBC Protection for Civilian, Beijing, 102205, China.
Bin DuState Key Laboratory of NBC Protection for Civilian, Beijing, 102205, China.
Zhaoyang TongState Key Laboratory of NBC Protection for Civilian, Beijing, 102205, China.
Jianjie XuState Key Laboratory of NBC Protection for Civilian, Beijing, 102205, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Quantitative analysis is essential for virus research, especially in determining the virus titer. The classical method plaque assay is time-consuming, complex, and difficult for the phages that cannot form apparent plaque on the solid medium. In order to realize rapid and effective detection, a new method combining atomic force microscopy (AFM) observation and mathematical calculation is established. In this research, M13 phages with an appropriate dilution ratio were observed and counted by AFM. Based on the counting results, the titer of M13 phages can be calculated simply through mathematical substitution. Instead of cultivating overnight in plaque assay, this new method can be implemented within a few hours. Moreover, it is a method that can achieve visualization for titer determination and have the potential to determine the phages that fail to form apparent plaque, which is significant in virus quantitative assessment.

Indexed as

AFMFail to form plaqueM13 bacteriophage titerMathematical deviationVisualization

Identifiers

PMID35891946
PMCPMC9293941
OpenAlexW4285405554

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.