Evidence map›Paper›PMID 35890206›Full record

ArticlePharmaceutics2022

Hybrid Polyelectrolyte Nanocomplexes for Non-Viral Gene Delivery with Favorable Efficacy and Safety Profile.

Gabriele Maiorano, Clara Guido, Annamaria Russo, Andrea Giglio, Loris Rizzello, Mariangela Testini, Barbara Cortese, Stefania D'Amone, Giuseppe Gigli, Ilaria Elena Palamà

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Pharmaceutics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.5field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 8 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 1 country.

Gabriele MaioranoNanotechnology Institute of National Research Council, CNR-NANOTEC, Monteroni Street, 73100 Lecce, Italy.ORCID 0000-0003-2205-2293
Clara GuidoNanotechnology Institute of National Research Council, CNR-NANOTEC, Monteroni Street, 73100 Lecce, Italy.
Annamaria RussoNanotechnology Institute of National Research Council, CNR-NANOTEC, Monteroni Street, 73100 Lecce, Italy.
Andrea GiglioNanotechnology Institute of National Research Council, CNR-NANOTEC, Monteroni Street, 73100 Lecce, Italy.ORCID 0000-0002-3937-913X
Loris RizzelloDepartment of Pharmaceutical Sciences (DISFARM), University of Milan, G. Balzaretti 9 Street, 20133 Milan, Italy.
Mariangela TestiniNanotechnology Institute of National Research Council, CNR-NANOTEC, Monteroni Street, 73100 Lecce, Italy.
Barbara CorteseNanotechnology Institute of National Research Council, CNR-NANOTEC, c/o La Sapienza University, Piazzale Aldo Moro, 00185 Rome, Italy.ORCID 0000-0001-8667-7255
Stefania D'AmoneNanotechnology Institute of National Research Council, CNR-NANOTEC, Monteroni Street, 73100 Lecce, Italy.
Giuseppe GigliNanotechnology Institute of National Research Council, CNR-NANOTEC, Monteroni Street, 73100 Lecce, Italy.
Ilaria Elena PalamàNanotechnology Institute of National Research Council, CNR-NANOTEC, Monteroni Street, 73100 Lecce, Italy.ORCID 0000-0003-4420-0680
Istituto di Nanotecnologia · ITUniversity of Salento · ITUniversity of Milan · IT

Funding

AIRC project under IG 2021 ID. 26328 project - P.I. Cortese BarbaraERC-2019-StG 850936Fondazione Cariplo 2019-4278Progetto PON ARS01_00906 "TITAN - Nanotecnologie per l'immunoterapia dei tumori" FESR nell'ambito del PON "Ricerca e Innovazione" 2014 - 2020 - Azione II - OS 1.bTecnopolo di Nanotecnologia e Fotonica per la medicina di precisione" (TECNOMED) FISR/MIUR-CNR: delibera CIPE n. 3449 del 7-08-2017Tecnopolo per la medicina di precisione" (TecnoMed Puglia) Regione Puglia: DGR n. 2117 del 21/11/2018
6 · The paper itself

Abstract

The development of nanovectors for precise gene therapy is increasingly focusing on avoiding uncontrolled inflammation while still being able to effectively act on the target sites. Herein, we explore the use of non-viral hybrid polyelectrolyte nanocomplexes (hPECs) for gene delivery, which display good transfection efficacy coupled with non-inflammatory properties. Monodisperse hPECs were produced through a layer-by-layer self-assembling of biocompatible and biodegradable polymers. The resulting nanocomplexes had an inner core characterized by an EGFP-encoding plasmid DNA (pDNA) complexed with linear polyethyleneimine or protamine (PEI or PRM) stabilized with lecithin and poly(vinyl alcohol) (PVA) and an outer layer consisting of medium-molecular-weight chitosan (CH) combined with tripolyphosphate (TPP). PEI- and PRM-hPECs were able to efficiently protect the genetic cargo from nucleases and to perform a stimuli-responsive release of pDNA overtime, thus guaranteeing optimal transfection efficiency. Importantly, hPECs revealed a highly cytocompatible and a non-inflammatory profile in vitro. These results were further supported by evidence of the weak and unspecific interactions of serum proteins with both hPECs, thus confirming the antifouling properties of their outer shell. Therefore, these hPECs represent promising candidates for the development of effective, safe nanotools for gene delivery.

Indexed as

hybrid nanovectornon-viral gene deliverysafety profile

Identifiers

PMID35890206
PMCPMC9323431
OpenAlexW4283312787

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.