ArticleNutrients2022
Regulation of Dietary Protein Solubility Improves Ruminal Nitrogen Metabolism In Vitro: Role of Bacteria-Protozoa Interactions.
Article in Nutrients, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.
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Who cites it
18 citing papers in PubMed, 1 synthesis or guideline pooled it, 30 citations in OpenAlex.
- Gut protozoa of wild rodents - a meta-analysis.Parasitology · 2024Pooled it
- Effects of fermented mulberry leaves on rumen fermentation and microbiota in rumen fluid of heat-stressed Hu sheep.Applied microbiology and biotechnology · 2026Article
- Genome-resolved multi-omics provide new insights into microbial nitrogen utilization by the rumen microbiota.Microbiome · 2026Article
- Fermentation quality and microbial communities of mixed silage from Pennisetum glaucum × purpureum and Rosa roxburghii residue.BMC microbiology · 2026Article
- Microbial niches at the One Health interface: linking environmental factors, biomes, and disease dynamics.Frontiers in microbiology · 2026Review
- Regulation ofToxins · 2025Article
- Hepatic antioxidant capacity, immune response, and glycolysis of Tibetan sheep in response to dietary soluble protein levels.Protoplasma · 2025Article
- The crosstalk between host and rumen microbiome in cattle: insights from multi-omics approaches and genome-wide association studies.World journal of microbiology & biotechnology · 2025Review
- Dietary Protein-Induced Changes in Archaeal Compositional Dynamics, Methanogenic Pathways, and Antimicrobial Resistance Profiles in Lactating Sheep.Microorganisms · 2025Article
- Reciprocal regulation of rumen microbiota and epithelial genes in response to small peptide supplementation for feed efficiency in beef cattle.Frontiers in veterinary science · 2025Article
- Hydrogen-rich water 400ppb as a potential strategy for improving ruminant nutrition and mitigating methane emissions.BMC microbiology · 2024Article
- Impacts of Protein and Energy Levels on Rumen Fermentation and Microbial Activity Under Different Incubation Temperatures.Animals : an open access journal from MDPI · 2024Article
- Dietary crude protein and protein solubility manipulation enhances intestinal nitrogen absorption and mitigates reactive nitrogen emissions through gut microbiota and metabolome reprogramming in sheep.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2024Article
- Effective microorganism combinations improve the quality of compost-bedded pack products in heifer barns: exploring pack bacteria-fungi interaction mechanisms.BMC microbiology · 2024Article
- Insights into Effects of Combined Capric and Lauric Acid on Rumen Bacterial Composition.Microorganisms · 2024Article
- Antioxidant Capacity, Inflammatory Response, Carcass Characteristics and Meat Quality of Hu Sheep in Response to Dietary Soluble Protein Levels with Decreased Crude Protein Content.Antioxidants (Basel, Switzerland) · 2023Article
- Integrative analysis of the gut microbiota and faecal and serum short-chain fatty acids and tryptophan metabolites in patients with cirrhosis and hepatic encephalopathy.Journal of translational medicine · 2023Article
- Mixed silage with Chinese cabbage waste enhances antioxidant ability by increasing ascorbate and aldarate metabolism through rumenFrontiers in microbiology · 2022Article
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Precision dietary interventions (e.g., altering proportions of dietary protein fractions) has significant implications for the efficiency of nutrient use in ruminants, as well as lowering their environmental footprint, specifically nitrogen (N) emissions. Soluble protein (SP) is defined as the protein fraction that is rapidly degraded in the rumen (e.g., non-protein N and true protein), and our previous study found that regulating SP levels could improve N efficiency in Hu sheep. Thus, the present study was conducted to explore in vitro how protein fractions with different SP levels modulate the rumen microbial community and its association with N metabolism. Four dietary treatments with different SP proportions and similar crude protein (CP) content (~14%) were formulated (% of CP): 20 (S20), 30 (S30), 40 (S40) and 50 (S50). Results showed that NH3-N content increased with increasing SP levels at 4, 12 and 24 h; TVFA, acetate, propionate and valerate were higher in S30 and S40 (p < 0.05) and had quadratic effects (p < 0.05). Moreover, dry matter digestibility (DMD) and N digestibility (ND) were all decreased with S20 and S50 (p < 0.05). The S30 and S40 treatments increased the abundance of Bacteroidetes and Prevotella (Prevotella_ruminicola) but decreased the abundance of Firmicutes and Proteobacteria (p < 0.05). Bacterial pathways related to amino acid and fatty acid metabolism also were enriched with S30 and S40. The abundance of Entodinium was increased with S30 and S40 and had a positive correlation with Prevotella, and these two genera also played an important role in N metabolism and VFA synthesis of this study. In conclusion, bacterial and protozoal communities were altered by the level of SP (% of CP), with higher SP levels (~50% of CP) increasing the microbial diversity but being detrimental to rumen N metabolism.
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