Protein Attachment Mechanism for Improved Functionalization of Affinity Monolith Chromatography (AMC).

Nayan Nayak, Rosalinda Mazzei, Lidietta Giorno, João G Crespo, Carla A M Portugal, Teresa Poerio

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In one paragraph

Article in Molecules (Basel, Switzerland), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

6 authors.

Nayan NayakLAQV-REQUIMTE, Department of Chemistry, NOVA School of Science and Technology, FCT NOVA, Universidade NOVA de Lisboa, 2829-516 Caparica, Portugal.ORCID 0000-0002-4975-7317

Rosalinda MazzeiInstitute on Membrane Technology, National Research Council, ITM-CNR, Via P. Bucci, 17/C, I87030 Rende, Italy.ORCID 0000-0001-9575-7310

Lidietta GiornoInstitute on Membrane Technology, National Research Council, ITM-CNR, Via P. Bucci, 17/C, I87030 Rende, Italy.ORCID 0000-0002-4408-5849

João G CrespoLAQV-REQUIMTE, Department of Chemistry, NOVA School of Science and Technology, FCT NOVA, Universidade NOVA de Lisboa, 2829-516 Caparica, Portugal.

Carla A M PortugalLAQV-REQUIMTE, Department of Chemistry, NOVA School of Science and Technology, FCT NOVA, Universidade NOVA de Lisboa, 2829-516 Caparica, Portugal.

Teresa PoerioInstitute on Membrane Technology, National Research Council, ITM-CNR, Via P. Bucci, 17/C, I87030 Rende, Italy.ORCID 0000-0001-8449-9344

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This work aims at understanding the attachment mechanisms and stability of proteins on a chromatography medium to develop more efficient functionalization methodologies, which can be exploited in affinity chromatography. In particular, the study was focused on the understanding of the attachment mechanisms of bovine serum albumin (BSA), used as a ligand model, and protein G on novel amine-modified alumina monoliths as a stationary phase. Protein G was used to develop a column for antibody purification. The results showed that, at lower protein concentrations (i.e., 0.5 to 1.0 mg·mL

Indexed as

AntibodiesSerum Albumin, BovineAdsorptionAminesChromatography, AffinityLigandsAminesAntibodiesLigandsSerum Albumin, BovineAPTES functionalizationchromatographyimmunoglobulin G purificationmonolithsprotein-binding kineticsprotein Gprotein immobilization

Identifiers

PMID35889369

DOI10.3390/molecules27144496

PMCPMC9323659

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