Evidence map›Paper›PMID 35887340›Full record

ArticleInternational journal of molecular sciences2022

A Preparative Method for the Isolation of Calponin from Molluscan Catch Muscle.

Stanislav S Lazarev, Ulyana V Shevchenko, Vyacheslav A Dyachuk, Ilya G Vyatchin

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.5field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Stanislav S LazarevLaboratory of Cell Biophysics, A.V. Zhirmunsky National Scientific Center of Marine Biology, Far Eastern Branch, Russian Academy of Sciences, 17 Palchevsky Str., 690041 Vladivostok, Russia.
Ulyana V ShevchenkoLaboratory of Cell Biophysics, A.V. Zhirmunsky National Scientific Center of Marine Biology, Far Eastern Branch, Russian Academy of Sciences, 17 Palchevsky Str., 690041 Vladivostok, Russia.
Vyacheslav A DyachukLaboratory of Cell Biophysics, A.V. Zhirmunsky National Scientific Center of Marine Biology, Far Eastern Branch, Russian Academy of Sciences, 17 Palchevsky Str., 690041 Vladivostok, Russia.ORCID 0000-0001-8100-7367
Ilya G VyatchinLaboratory of Cell Biophysics, A.V. Zhirmunsky National Scientific Center of Marine Biology, Far Eastern Branch, Russian Academy of Sciences, 17 Palchevsky Str., 690041 Vladivostok, Russia.
A.V. Zhirmunsky National Scientific Center of Marine Biology Far Eastern Branch of the Russian Academy of Sciences · RU

Funding

Russian Foundation for Basic Research 19-04-00991
6 · The paper itself

Abstract

We describe the development of a preparative method to isolate molluscan catch muscle, calponin. This method is based on the ability of calponin to interact with actin in a temperature-dependent manner. After extracting thin filaments, as previously described, the extract was ultracentrifuged at 2 °C. While other surface proteins of thin filaments co-precipitated with actin, calponin, along with some minor contaminants, remained in the supernatant. Calponin was purified through cation-exchange chromatography. The yield of pure protein was four-fold higher than that achieved through high-temperature extraction. To evaluate functionally isolated proteins, we determined the effect of calponin on Mg

Indexed as

ActinsActomyosinCalcium-Binding ProteinsCalponinsMicrofilament ProteinsMuscle, SmoothMyosinsActinsActomyosinCalcium-Binding ProteinsCalponinsMicrofilament ProteinsMyosinsactin-activated myosin Mg2+-ATPase activitycatch muscle calponin extractionmolluscan catch musclesmolluscan thin filaments

Identifiers

PMID35887340
PMCPMC9315827
OpenAlexW4286005882

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.