Evidence map›Paper›PMID 35886947›Full record

ArticleInternational journal of molecular sciences2022

Structural Analysis of Ino2p/Ino4p Mutual Interactions and Their Binding Interface with Promoter DNA.

Muhammad Hidayatullah Khan, Lu Xue, Jian Yue, Hans-Joachim Schüller, Zhongliang Zhu, Liwen Niu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Muhammad Hidayatullah KhanHefei National Laboratory for Physical Sciences at the Microscale, Division of Molecular and Cellular Biophysics, University of Science and Technology of China, Hefei 230026, China.ORCID 0000-0003-1703-164X
Lu XueHefei National Laboratory for Physical Sciences at the Microscale, Division of Molecular and Cellular Biophysics, University of Science and Technology of China, Hefei 230026, China.
Jian YueHefei National Laboratory for Physical Sciences at the Microscale, Division of Molecular and Cellular Biophysics, University of Science and Technology of China, Hefei 230026, China.
Hans-Joachim SchüllerInstitut für Genetik und Funktionelle Genomforschung, Felix-Hausdorff-Str. 8, 17487 Greifswald, Germany.
Zhongliang ZhuHefei National Laboratory for Physical Sciences at the Microscale, Division of Molecular and Cellular Biophysics, University of Science and Technology of China, Hefei 230026, China.
Liwen NiuHefei National Laboratory for Physical Sciences at the Microscale, Division of Molecular and Cellular Biophysics, University of Science and Technology of China, Hefei 230026, China.
University of Science and Technology of China · CN

Funding

Ministry of Science and Technology of China 2017YFA0504903National Natural Science Foundation of China 31621002National Natural Science Foundation of China 92153302Strategic Priority Research Program of the Chinese Academy of Sciences XDB37010000
6 · The paper itself

Abstract

Gene expression is mediated by a series of regulatory proteins, i.e., transcription factors. Under different growth conditions, the transcriptional regulation of structural genes is associated with the recognition of specific regulatory elements (REs) in promoter DNA. The manner by which transcription factors recognize distinctive REs is a key question in structural biology. Previous research has demonstrated that Ino2p/Ino4p heterodimer is associated with the transcriptional regulation of phospholipid biosynthetic genes. Mechanistically, Ino2p/Ino4p could specifically recognize the inositol/choline-responsive element (ICRE), followed by the transcription activation of the phospholipid biosynthetic gene. While the promoter DNA sequence for Ino2p has already been characterized, the structural basis for the mutual interaction between Ino2p/Ino4p and their binding interface with promoter DNA remain relatively unexplored. Here, we have determined the crystalline structure of the Ino2p

Indexed as

Basic Helix-Loop-Helix ProteinsGene Expression Regulation, FungalPromoter Regions, GeneticSaccharomyces cerevisiae ProteinsTranscription FactorsDNAPhospholipidsSaccharomyces cerevisiaeBasic Helix-Loop-Helix ProteinsDNAINO2 protein, S cerevisiaeINO4 protein, S cerevisiaePhospholipidsSaccharomyces cerevisiae ProteinsTranscription Factorsbasic helix–loop–helix (bHLH)Ino2p/Ino4p heterodimerinterprotein–DNA binding patternprotein–protein mutual interactionresponsive elements (RE)

Identifiers

PMID35886947
PMCPMC9315497
OpenAlexW4285009338

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.