ArticleBiosensors2022
Novel Amperometric Biosensor Based on Tyrosinase/Chitosan Nanoparticles for Sensitive and Interference-Free Detection of Total Catecholamine.
Article in Biosensors, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 26 citations in OpenAlex.
- Electro-Biocatalytic Reactivity of Catecholamine at a Lignin Nanoparticle-Tyrosinase Interface.Molecules (Basel, Switzerland) · 2026Article
- 3D-Printed PLA Hollow Microneedles Loaded with Chitosan Nanoparticles for Colorimetric Glucose Detection in Sweat Using Machine Learning.Biosensors · 2025Article
- Towards Precision Nutrition: A Novel Smartphone-Connected Biosensor for Point-of-Care Detection of β-Hydroxybutyrate in Human Blood and Saliva.Sensors (Basel, Switzerland) · 2025Article
- Synthesis of Chitosan Nanoparticles via Microfluidic Approach: The Role of Temperature in Tailoring Aggregation for Enhanced Uniformity.Micromachines · 2025Article
- Recent Advances in Amperometric Biosensors for Medical Applications: A Mini-Review.Current topics in medicinal chemistry · 2025Review
- Sensing the Future-Frontiers in Biosensors: Exploring Classifications, Principles, and Recent Advances.ACS omega · 2024Review
- An Updated Review on Electrochemical Nanobiosensors for Neurotransmitter Detection.Biosensors · 2023Review
- Laser Scribing Turns Plastic Waste into a Biosensor via the Restructuration of Nanocarbon Composites for Noninvasive Dopamine Detection.Biosensors · 2023Article
- Enzymatic Electrochemical/Fluorescent Nanobiosensor for Detection of Small Chemicals.Biosensors · 2023Review
- Disposable Voltammetric Immunosensor for D-Dimer Detection as Early Biomarker of Thromboembolic Disease and of COVID-19 Prognosis.Biosensors · 2022Article
- A Novel Paper-Based Electrochemical Biosensor Based on N,O-Rich Covalent Organic Frameworks for Carbaryl Detection.Biosensors · 2022Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
The regulation of nervous and cardiovascular systems and some brain-related behaviors, such as stress, panic, anxiety, and depression, are strictly dependent on the levels of the main catecholamines of clinical interest, dopamine (DA), epinephrine (EP), and norepinephrine (NEP). Therefore, there is an urgent need for a reliable sensing device able to accurately monitor them in biological fluids for early diagnosis of the diseases related to their abnormal levels. In this paper, we present the first tyrosinase (Tyr)-based biosensor based on chitosan nanoparticles (ChitNPs) for total catecholamine (CA) detection in human urine samples. ChitNPs were synthetized according to an ionic gelation process and successively characterized by SEM and EDX techniques. The screen-printed graphene electrode was prepared by a two-step drop-casting method of: (i) ChitNPS; and (ii) Tyr enzyme. Optimization of the electrochemical platform was performed in terms of the loading method of Tyr on ChitNPs (nanoprecipitation and layer-by-layer), enzyme concentration, and enzyme immobilization with and without 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) and N-hydroxysuccinimide (NHS) as cross-linking agents. The Tyr/EDC-NHS/ChitNPs nanocomposite showed good conductivity and biocompatibility with Tyr enzyme, as evidenced by its high biocatalytic activity toward the oxidation of DA, EP, and NEP to the relative o-quinone derivatives electrochemically reduced at the modified electrode. The resulting Tyr/EDC-NHS/ChitNPs-based biosensor performs interference-free total catecholamine detection, expressed as a DA concentration, with a very low LOD of 0.17 μM, an excellent sensitivity of 0.583 μA μM
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.