Evidence map›Paper›PMID 35883816›Full record

ArticleAntioxidants (Basel, Switzerland)2022

Glycosylation of Epigallocatechin Gallate by Engineered Glycoside Hydrolases from

Juan A Méndez-Líter, Ana Pozo-Rodríguez, Enrique Madruga, María Rubert, Andrés G Santana, Laura I de Eugenio, Cristina Sánchez, Ana Martínez, Alicia Prieto, María Jesús Martínez

Open access · goldAbstract read
In one paragraph

Article in Antioxidants (Basel, Switzerland), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
1.3field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
  4. Article
  5. Article
  6. Article
  7. Review
  8. Bioavailability and Bioactivity of Plant Antioxidants.Antioxidants (Basel, Switzerland) · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Juan A Méndez-LíterCentro de Investigaciones Biológicas Margarita Salas, Department of Microbial and Plant Biotechnology, Spanish National Research Council (CSIC), Ramiro de Maeztu 9, 28040 Madrid, Spain.
Ana Pozo-RodríguezCentro de Investigaciones Biológicas Margarita Salas, Department of Microbial and Plant Biotechnology, Spanish National Research Council (CSIC), Ramiro de Maeztu 9, 28040 Madrid, Spain.ORCID 0000-0003-1524-4131
Enrique MadrugaCentro de Investigaciones Biológicas Margarita Salas, Department of Structural and Chemical Biology, Spanish National Research Council (CSIC), Ramiro de Maeztu 9, 28040 Madrid, Spain.ORCID 0000-0002-7012-9737
María RubertDepartment of Biochemistry and Molecular Biology, School of Biology, Instituto de Investigación Hospital 12 de Octubre, Universidad Complutense de Madrid, C/de José Antonio Nováis 12, 28040 Madrid, Spain.
Andrés G SantanaDepartment of Bioorganic Chemistry, Instituto de Química Orgánica General, Spanish National Research Council (CSIC), C/Juan de la Cierva 3, 28006 Madrid, Spain.ORCID 0000-0003-3568-7714
Laura I de EugenioCentro de Investigaciones Biológicas Margarita Salas, Department of Microbial and Plant Biotechnology, Spanish National Research Council (CSIC), Ramiro de Maeztu 9, 28040 Madrid, Spain.
Cristina SánchezDepartment of Biochemistry and Molecular Biology, School of Biology, Instituto de Investigación Hospital 12 de Octubre, Universidad Complutense de Madrid, C/de José Antonio Nováis 12, 28040 Madrid, Spain.ORCID 0000-0002-1428-3078
Ana MartínezCentro de Investigaciones Biológicas Margarita Salas, Department of Structural and Chemical Biology, Spanish National Research Council (CSIC), Ramiro de Maeztu 9, 28040 Madrid, Spain.ORCID 0000-0002-2707-8110
Alicia PrietoCentro de Investigaciones Biológicas Margarita Salas, Department of Microbial and Plant Biotechnology, Spanish National Research Council (CSIC), Ramiro de Maeztu 9, 28040 Madrid, Spain.ORCID 0000-0002-5075-4025
María Jesús MartínezCentro de Investigaciones Biológicas Margarita Salas, Department of Microbial and Plant Biotechnology, Spanish National Research Council (CSIC), Ramiro de Maeztu 9, 28040 Madrid, Spain.ORCID 0000-0003-2166-1097
Consejo Superior de Investigaciones Científicas · ESUniversidad Complutense de Madrid · ES

Funding

Comunidad de Madrid RETOPROSOST-2-CM P2018/EMT-4459CSIC Interdisciplinary Thematic Platform PTI-NEURO-AGING+Instituto de Salud Carlos III PI20/00590MICIU/AEI/FEDER RTI2018-093683-B-I00
6 · The paper itself

Abstract

Glycoside hydrolases (GHs) are enzymes that hydrolyze glycosidic bonds, but some of them can also catalyze the synthesis of glycosides by transglycosylation. However, the yields of this reaction are generally low since the glycosides formed end up being hydrolyzed by these same enzymes. For this reason, mutagenic variants with null or drastically reduced hydrolytic activity have been developed, thus enhancing their synthetic ability. Two mutagenic variants, a glycosynthase engineered from a β-glucosidase (BGL-1-E521G) and a thioglycoligase from a β-xylosidase (BxTW1-E495A), both from the ascomycete

Indexed as

fungal enzymesglucoseglycoconjugatespolyphenolsophorosexylose

Identifiers

PMID35883816
PMCPMC9312355
OpenAlexW4283832288

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.