Evidence map›Paper›PMID 35883301›Full record

ArticleAnimals : an open access journal from MDPI2022

The Development of a Multiplex Real-Time Quantitative PCR Assay for the Differential Detection of the Wild-Type Strain and the MGF505-2R, EP402R and I177L Gene-Deleted Strain of the African Swine Fever Virus.

Kang Zhao, Kaichuang Shi, Qingan Zhou, Chenyong Xiong, Shenglan Mo, Hongjin Zhou, Feng Long, Haina Wei, Liping Hu, Meilan Mo

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Animals : an open access journal from MDPI, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
5.4field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 24 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Kang ZhaoCollege of Animal Science and Technology, Guangxi University, Nanning 530005, China.ORCID 0000-0003-3568-8231
Kaichuang ShiCollege of Animal Science and Technology, Guangxi University, Nanning 530005, China.
Qingan ZhouGuangxi Center for Animal Disease Control and Prevention, Nanning 530001, China.
Chenyong XiongCollege of Animal Science and Technology, Guangxi University, Nanning 530005, China.
Shenglan MoGuangxi Center for Animal Disease Control and Prevention, Nanning 530001, China.
Hongjin ZhouCollege of Animal Science and Technology, Guangxi University, Nanning 530005, China.
Feng LongGuangxi Center for Animal Disease Control and Prevention, Nanning 530001, China.
Haina WeiGuangxi Center for Animal Disease Control and Prevention, Nanning 530001, China.
Liping HuGuangxi Center for Animal Disease Control and Prevention, Nanning 530001, China.
Meilan MoCollege of Animal Science and Technology, Guangxi University, Nanning 530005, China.ORCID 0000-0003-3903-0509
Guangxi Center for Disease Prevention and Control · CNGuangxi University · CN

Funding

the Key Research and Development Program, and the Science and Technology Major Project of Guangxi Science and Technology Bureau, China AB21238003; AA17204057
6 · The paper itself

Abstract

African swine fever virus (ASFV) causes African swine fever (ASF), a devastating hemorrhagic disease of domestic pigs and wild boars. Currently, the MGF505R, EP402R (CD2v) and I177L gene-deleted ASFV strains were confirmed to be the ideal vaccine candidate strains. To develop an assay for differentiating the wild-type and gene-deleted ASFV strains, four pairs of specific primers and TaqMan probes targeting the ASFV B646L (p72), I177L, MGF505-2R and EP402R (CD2v) genes were designed. A multiplex real-time qPCR assay for the differential detection of the wild-type and gene-deleted ASFV strains was developed after optimizing the reaction conditions, including the annealing temperature, primer concentration and probe concentration. The results showed that the multiplex real-time qPCR assay can specifically test the ASFV B646L (p72), I177L, MGF505-2R and EP402R (CD2v) genes with a limit of detection (LOD) of 32.1 copies/μL for the B646L (p72) gene, and 3.21 copies/μL for the I177L, MGF505-2R and EP402R (CD2v) genes. However, the assay cannot test for the classical swine fever virus (CSFV), porcine reproductive and respiratory syndrome virus (PRRSV), porcine epidemic diarrhea virus (PEDV), porcine deltacoronavirus (PDCoV), porcine circovirus type 2 (PCV2), PCV3 and pseudorabies virus (PRV). The assay demonstrated good repeatability and reproducibility with coefficients of variation (CV) less than 1.56% for both the intra- and inter-assay. The assay was used to test 4239 clinical samples, and the results showed that 12.60% (534/4239) samples were positive for ASFV, of which 10 samples lacked the EP402R gene, 6 samples lacked the MGF505-2R gene and 14 samples lacked the EP402R and MGF505-2R genes. The results indicated that the multiplex real-time qPCR developed in this study can provide a rapid, sensitive and specific diagnostic tool for the differential detection of the ASFV B646L, I177L, MGF505-2R and EP402R genes.

Indexed as

African swine fever virus (ASFV)detectiondifferentiationgene-deleted strainmultiplex real-time qPCRwild-type strain

Identifiers

PMID35883301
PMCPMC9311895
OpenAlexW4284969294

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.