Evidence map›Paper›PMID 35881363›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Visualization of Infected Red Blood Cell Surface Antigens by Fluorescence Microscopy.

Markus Ganter, Julien Guizetti, Nicole Kilian

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
4.1field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Markus GanterCentre for Infectious Diseases, Parasitology, Heidelberg University Hospital, Heidelberg, Germany. Markus.Ganter@med.uni-heidelberg.de.ORCID 0000-0003-0684-2260
Julien GuizettiCentre for Infectious Diseases, Parasitology, Heidelberg University Hospital, Heidelberg, Germany.ORCID 0000-0002-8762-4259
Nicole KilianCentre for Infectious Diseases, Parasitology, Heidelberg University Hospital, Heidelberg, Germany.ORCID 0000-0001-7489-9782
Heidelberg University · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Immunofluorescence labeling enables the detection and characterization of various parasite proteins presented on the surface of the infected red blood cell. Several approaches for immunofluorescence detection of red blood cell surface-presented proteins of Plasmodium spp. have been successfully established and published over the years. However, finding the right approach depends on the scientific question, and different protocols have different advantages. Here, we discuss some aspects that should be considered and present an easily applicable protocol for labeling parasite surface antigens, which subsequently can be analyzed by immunofluorescence microscopy (or flow cytometry).

Indexed as

ErythrocytesPlasmodium falciparumAntigens, SurfaceMembrane ProteinsMicroscopy, FluorescenceStaining and LabelingAntigens, SurfaceMembrane ProteinsCytoadhesionFixationImmunofluorescenceInfected red blood cellsMicroscopySTED super-resolution microscopy

Identifiers

PMID35881363
OpenAlexW4288062147

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.