Evidence map›Paper›PMID 35881358›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Protocol for Differential Biopanning of P. falciparum Phage Display cDNA Library to Identify Parasite Targets of Protective Antibodies.

Jenna Zuromski, Jonathan Kurtis, Dipak Kumar Raj

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.5field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Jenna ZuromskiDepartment of Pathology and Laboratory Medicine, Brown University Medical School, Providence, RI, USA.ORCID 0000-0001-6234-5854
Jonathan KurtisDepartment of Pathology and Laboratory Medicine, Brown University Medical School, Providence, RI, USA.
Dipak Kumar RajDepartment of Pathology and Laboratory Medicine, Brown University Medical School, Providence, RI, USA. Dipak_Raj@Brown.edu.
Brown University · US

Funding

Tfh responses to novel vaccine candidates and protection from pediatric falciparum malariaR01AI127699 · NIAID · BROWN UNIVERSITY · PI KURTIS, JONATHAN D. · 2017 to 2021
$3.4M
PfSEA-1 based vaccines for falciparum malariaR01AI110699 · NIAID · RHODE ISLAND HOSPITAL · PI KURTIS, JONATHAN D. · 2014 to 2018
$2.7M
Targeted Vaccine Development for Pediatric Falciparum MalariaR01AI076353 · NIAID · RHODE ISLAND HOSPITAL · PI KURTIS, JONATHAN D. · 2008 to 2011
$2.4M
NIAID NIH HHS R01 AI076353NIAID NIH HHS R01 AI110699NIAID NIH HHS R01 AI127699
6 · The paper itself

Abstract

Malaria remains a significant global health burden, killing hundreds of thousands of children annually (WHO, The world malaria report. WHO, Geneva, 2019). Despite decades of effort, no broadly effective vaccine exists. Differential screening of parasite phage display libraries is a promising approach to identify the targets of human antibodies expressed by resistant but not by susceptible individuals (Raj et al., Nature, 582, 104-108, 2020; Science, 344, 871-877, 2014). Our whole proteome differential screening (WPDS) approach consists of positive selection to capture phage that bind antibodies expressed by malaria-resistant individuals, followed by negative selection to remove phage that bind antibodies expressed by malaria-susceptible individuals, and amplification of differentially recognized clones.

Indexed as

BacteriophagesMalariaMalaria, FalciparumParasitesAnimalsAntibodies, ProtozoanBioprospectingChildGene LibraryHumansPeptide LibraryPlasmodium falciparumProtozoan ProteinsAntibodies, ProtozoanPeptide LibraryProtozoan ProteinsBiopanningBlood-stage malaria parasiteMalaria-resistant childrenPediatric malaria vaccinePlasmodium falciparum cDNA libraryT7 phage display

Identifiers

PMID35881358
OpenAlexW4288061781

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.